Assays & Kits

Chalcone isomerase (CHI) is the first recognized enzyme related to flavonoid synthesis and one of the key enzymes in flavonoid metabolism. Chalcone isomerase and chalcone synthase together constitute rate-limiting enzymes for flavonoid biosynthesis. CheKine™ Micro Chalcone Isomerase (CHI) Activity Assay Kit can detect plant tissues samples. In this kit, CHI catalyzed the cyclization of chalcone to form 4,5,7-trihydroxyflavanones, and the activity of CHI was indicated by measuring the absorbance change at 381 nm.

Malic enzyme (ME) is widely present in the cytoplasm of microorganisms, cultured cells, animals, and plants, especially with high activity in plant tissues. ME catalyzes the reversible oxidation decarboxylation of malic acid, producing pyruvate and CO2, as well as the reduction reaction accompanied by NAD(P)+, which is a key enzyme in malic acid metabolism. ME activity is closely related to biosynthesis and antioxidant activity. Based on the specificity of coenzyme and substrate, ME can be divided into NAD-ME (EC1.1.1.38) and NADP-ME (EC1.1.1.40). NAD-ME can catalyze the reduction of NAD+ to NADH, and the rate of NADH increase at 340 nm can reflect its activity.

SucrosePhosphorylase (SP, EC2.4.1.7) is mainly present in microorganisms and plants and belongs to the glycosylhydrolase 13 family. It is an enzyme that catalyzes the transfer of glucoside bonds and can catalyze the synthesis of 1-phospho-glucose from sucrose and inorganic phosphate. This enzyme mainly uses sucrose and glucose 1-phosphate as donors, and many substances such as polyhydroxyl sugars, sugar alcohols, phenolic hydroxyl groups, carboxyl groups as receptors to catalyze the synthesis of various glycosides. CheKine™ Micro Sucrose Phosphorylase (SP) Activity Assay Kit can detect plant tissues, fungus samples. In this kit, SP can catalyze sucrose to produce glucose 1-phosphate, which is modified to glucose 6-phosphate under the catalysis of glucose phosphomutase, and reduce NADP+ to generate NADPH under the action of glucose 6-phosphate dehydrogenase, resulting in an increase in the light absorption value of 340nm. The SP activity was reflected by the increase rate of 340nm abs

PYROSTAR NEO+ IS A NEW ENDOTOXIN DETECTION REAGENT DEVELOPED BY RECOMBINANT TECHNOLOGY. COMBINE WITH TRADITIONAL LYSATE REAGENT AND RECOMBINANT 3 FACTORS (FACTOR C, FACTOR B, PROCLOTTING ENZYME) FROM LIMULUS POLYPHEMUS. IT IS A HIGH SENSITIVE COLORIMETRIC ASSAY SPECIFIC FOR ENDOTOXIN.

PYROGENS & ENDOTOXIN DETECTION AT FUJIFIM PYROGENS ARE FEVER-INDUCING SUBSTANCES THAT IF ADMINISTERED TO HUMANS ABOVE CERTAIN CONCENTRATIONS MAY CAUSE SEVERE INFLAMMATION AND IN SOME CASES EVEN LEAD TO DEATH. WHILE ENDOGENOUS PYROGENS MAINLY CONSIST OF CYTOKINES, EXOGENOUS PYROGENS HAVE SIGNIFICANTLY GREATER STRUCTURAL DIVERSITY, COMPRISING A BROAD RANGE OF DIFFERENT MOLECULES SUCH AS LIPOPOLYSACCHARIDES, GLUCANES OR SMALL ORGANIC COMPOUNDS SUCH AS 2,4-DINITROPHENOL. AMONG EXOGENOUS PYROGENS THE GRAM-NEGATIVE CELL-WALL COMPONENT LIPOPOLYSACCHARIDE (LPS) IS GENERALLY CONSIDERED AS BIOLOGICALLY MOST ACTIVE. LPS CONSIST OF LARGE GLYCOCONJUGATES, INCLUDING A LIPIDIC MOIETY AND A POLYSACCHARIDE CORE TERMED “O-ANTIGEN”. DUE TO THEIR TOXIC PROPERTIES, THEY ARE USUALLY REFERRED TO AS ENDOTOXINS. WHEN ENTERING THE BLOODSTREAM, ENDOTOXINS QUICKLY INTERACT WITH IMMUNE CELLS AND INDUCE STRONG INFLAMMATORY REACTION, RESULTING IN AN INCREASE OF THE BODY TEMPERATURE. OWING TO THEIR GREAT HEAT RESI

THE LIMULUS COLOR KY SERIES INCLUDES BOTH A MULTI-TEST KIT AND A SINGLE-TEST KIT, EACH DESIGNED FOR TIME-BASED CHROMOGENIC ANALYSIS, USING A SYNTHETIC SUBSTRATE WHICH PRODUCES A YELLOW COLOR AND CAN SPECIFICALLY DETECT ENDOTOXIN WITH HIGH SENSITIVITY. PRODUCT FEATURES: • ENDOTOXIN-SPECIFIC LYSATE, AVOIDS FALSE POSITIVE RESULTS FROM GLUCANS • AVAILABLE IN MULTI-TESTS VIALS OR SINGLE-TEST VIALS • QUANTITATIVE KINETIC-CHROMOGENIC ASSAY (KCA) REAGENT • KCA ASSAYS CAN BE PERFORMED IN TUBE READER OR MICROPLATE READER • KCA QUANTITATIVE RANGE DETECTION LIMIT OF 0.0002 EU/ML (SINGLE-TYPE) AND 0.0005 EU/ML (MULTI-TYPE). • AVAILABLE WITH MATCHED CONTROL STANDARD ENDOTOXIN (CSE) • 100UL SAMPLE SIZE WHEN USED WITH TUBE READER; 50UL SAMPLE SIZE WHEN USED WITH MICROPLATE READER

THE LIMULUS COLOR KY SERIES INCLUDES BOTH A MULTI-TEST KIT AND A SINGLE-TEST KIT, EACH DESIGNED FOR TIME-BASED CHROMOGENIC ANALYSIS, USING A SYNTHETIC SUBSTRATE WHICH PRODUCES A YELLOW COLOR AND CAN SPECIFICALLY DETECT ENDOTOXIN WITH HIGH SENSITIVITY. PRODUCT FEATURES: • ENDOTOXIN-SPECIFIC LYSATE, AVOIDS FALSE POSITIVE RESULTS FROM GLUCANS • AVAILABLE IN MULTI-TESTS VIALS OR SINGLE-TEST VIALS • QUANTITATIVE KINETIC-CHROMOGENIC ASSAY (KCA) REAGENT • KCA ASSAYS CAN BE PERFORMED IN TUBE READER OR MICROPLATE READER • KCA QUANTITATIVE RANGE DETECTION LIMIT OF 0.0002 EU/ML (SINGLE-TYPE) AND 0.0005 EU/ML (MULTI-TYPE). • AVAILABLE WITH MATCHED CONTROL STANDARD ENDOTOXIN (CSE) • 100UL SAMPLE SIZE WHEN USED WITH TUBE READER; 50UL SAMPLE SIZE WHEN USED WITH MICROPLATE READER

PYROGENS & ENDOTOXIN DETECTION AT FUJIFIM PYROGENS ARE FEVER-INDUCING SUBSTANCES THAT IF ADMINISTERED TO HUMANS ABOVE CERTAIN CONCENTRATIONS MAY CAUSE SEVERE INFLAMMATION AND IN SOME CASES EVEN LEAD TO DEATH. WHILE ENDOGENOUS PYROGENS MAINLY CONSIST OF CYTOKINES, EXOGENOUS PYROGENS HAVE SIGNIFICANTLY GREATER STRUCTURAL DIVERSITY, COMPRISING A BROAD RANGE OF DIFFERENT MOLECULES SUCH AS LIPOPOLYSACCHARIDES, GLUCANES OR SMALL ORGANIC COMPOUNDS SUCH AS 2,4-DINITROPHENOL. AMONG EXOGENOUS PYROGENS THE GRAM-NEGATIVE CELL-WALL COMPONENT LIPOPOLYSACCHARIDE (LPS) IS GENERALLY CONSIDERED AS BIOLOGICALLY MOST ACTIVE. LPS CONSIST OF LARGE GLYCOCONJUGATES, INCLUDING A LIPIDIC MOIETY AND A POLYSACCHARIDE CORE TERMED “O-ANTIGEN”. DUE TO THEIR TOXIC PROPERTIES, THEY ARE USUALLY REFERRED TO AS ENDOTOXINS. WHEN ENTERING THE BLOODSTREAM, ENDOTOXINS QUICKLY INTERACT WITH IMMUNE CELLS AND INDUCE STRONG INFLAMMATORY REACTION, RESULTING IN AN INCREASE OF THE BODY TEMPERATURE. OWING TO THEIR GREAT HEAT RESI

used to obtain microRNA from human K562 cells