Assays & Kits

Detect SA-β-Gal levels, Compatible with co-staining with immunostaining, Enable multiple staining with common green and red dyes

The iQuant™ microRNA Assay Kit is ideal for quantification of small RNA, including microRNA and siRNA, and both single stranded and double stranded RNA. The assay kit is highly selective for small RNA over rRNA or large mRNA. The assay kit offers advantages in stability, linear dynamic range, and sensitivity over other traditional of RNA quantitation. The assay kit contains concentrated quantitation reagent, dilution buffer, and pre-diluted microRNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the fluorescence using fluorescence plate reader or Fluorometer (Figure 1). The assay is well tolerated to common contaminants such as proteins, salts, solvents, detergents, or free nucleotides. The assay can be adapted for use in microplates, tubes or cuvettes.

The TR-FRET In vitro E3 activity kit has been developed to be simple, fast, and easy to use while being robust and sensitive. As E3 ligases autoubiquitinate they build long polyubiquitin chains; our unique assay will detect these chains as they are being built. This assay is homogenous and compatible for High-throughput Screening (HTS). Choose 1 E2 option among: UBE2D3 (UbcH5c), UBE2L3 (UbcH7), UBE2K (UbcH1), Ubc13, Mms2 (yeast), UBE2D2 (UbcH5b, E2-17K2), UBE2R1 (CDC34), UBE2R2 (Ubc3B), Non-tagged UBE2D1, His6-SUMO tagged UBE2D1, UBE2E2 (UbcH8), UBE2E3 (UbcH9), His6-tagged UBE2H (E2-20K), His6-SUMO tagged UBE2H (E2-20K), UBE2F (NCE2), UBE2M (Ubc12), UBE2N (Ubc13), Non-tagged UBE2T (PIG50), His6-SUMO tagged UBE2T (PIG50), UBE2V2 (MMS2, UEV-2), UBE2S (E2EPF), UBE2C (Ubc10), His6-tagged UBE2W (Ubc 16), UBE2Q2, His6-tagged UBE2A, Non-tagged UBE2G2 (Ubc7), His6-SUMO tagged UBE2G2 (Ubc7), His6-tagged UBE2I (Ubc9), UBE2L6 (UbcH8), UBE2Z (Use1) And Choose 1 E3 option among: ITCH, Traf6, GP78,

The Fc (IgG1): FcRn Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling neutralizing antibodies or inhibitors of the interaction between Fc (IgG1) and human FcRn.  The assay requires only a few steps. First, Fc (IgG1) is coated on a 96-well plate overnight. After blocking, the protein is pre-incubated with the inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-FcRn, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can be quenched and measured using a UV/Vis microplate reader.

The Fc (IgG1): FcRn Inhibitor Screening Colorimetric Assay Kit is designed for the screening and profiling of neutralizing antibodies or inhibitors of the interaction between human Fc (IgG1) and human FcRn (Neonatal Fc receptor for IgG). This kit comes in a convenient 96-well or 384-well format, with purified Biotinylated-FcRn complex (Fc receptor amino acids 24-297 and B2M amino acids 21-119) and Fc (IgG1) (amino acids 100-330) proteins, Streptavidin-HRP, and assay buffers for 100 or 400 reactions. A 96-well plate is coated with Fc (IgG1) protein. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. Upon subsequent incubation with FcRn-biotin, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can be quenched and measured using a UV/Vis microplate reader. The signal is proportional to the binding of FcRn to Fc (IgG1).

The WRN Helicase Activity Assay is a fluorogenic assay designed for screening and profiling of WRN (Werner Syndrome ATP-dependent Helicase) antagonists/inhibitors by monitoring their effect on WRN-catalyzed DNA unwinding. WRN inhibitor NCGC00063279 is also included as a control. The DNA probe is conjugated on one strand with the TAMRA (tetramethylrhodamine) fluorophore, and on the other strand with BHQ (Black Hole Quencher) which effectively quenches TAMRA fluorescence due to their proximity within the DNA double strand. WRN unwinding of the DNA probe separates the two strands, releasing TAMRA fluorescence. WRN activity, therefore, results in a proportional increase in fluorescence.

The WRN Helicase Activity Assay is a fluorogenic assay designed for screening and profiling of WRN (Werner Syndrome ATP-dependent Helicase) antagonists/inhibitors by monitoring their effect on WRN-catalyzed DNA unwinding. WRN Helicase Activity Assay Kit comes in a convenient 384-well format, with contains enough purified recombinant WRN, ATP, DNA substrate, assay buffer and additives for 400 reactions. The DNA probe is conjugated on one strand with the TAMRA (tetramethylrhodamine) fluorophore, and on the other strand with BHQ (Black Hole Quencher) which effectively quenches TAMRA fluorescence due to their proximity within the DNA double strand. WRN unwinding of the DNA probe separates the two strands, releasing TAMRA fluorescence. WRN activity, therefore, results in a proportional increase in fluorescence.

The PDE6C Assay Kit is a fluorescence polarization (FP), homogeneous, 384-well assay kit designed for the screening and profiling of PDE6C (Phosphodiesterase 6C) inhibitors. This assay takes advantage of a specific fluorescent phosphate-binding nanoparticle. The kit contains enough purified recombinant PDE6C, fluorescent probe, PDE assay buffer, Binding Agent, and diluent for 400 reactions. The assay uses a fluorescein-labeled cyclic guanosine monophosphate (cGMP-FAM for PDE6C), in which the phosphate group is engaged within the cyclic nucleotide. This is a very small molecule that rotates fast (low FP). PDE6C catalyzes the hydrolysis of the phosphodiester bond in the cyclic nucleotide and frees the phosphate group. In a second step the free phosphate group is recognized by a specific phosphate-binding nanobead (Binding Agent) leading to the formation of a large complex, with restricted movement (high FP). FP is proportional to PDE6C activity. This assay requires a fluorescent micropl