Assays & Kits

The Molecular Glue/PROTAC® Optimization Kit for RBM39-DCAF15 Binding is designed for the testing and profiling of Molecular Glues (MG)/PROTACs targeting the RBM39 (RNA-binding protein 39) and DCAF15 (DDB1 and CUL4 associated factor 15) interaction. The Molecular Glue/PROTAC® Optimization Kit for RBM39-DCAF15 Binding comes in a convenient AlphaLISA® format, with enough optimized assay and detection buffer, purified recombinant RBM39 (amino acids 245-530) and Rbx1/CUL4B/DDB1/DCAF15/DDA1 complex for 384 reactions. This kit also contains the molecular glue E7820 and Arginine as controls.

The GPX4 Fluorogenic Assay Kit is a fluorogenic assay designed to measure GPX4 (glutathione peroxidase 4) activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough purified recombinant GPX4, Glutathione, Cumene Hydroperoxide, NADPH, Glutathione Reductase, and GPX4 Assay Buffer for 100 reactions. Figure 1: Mechanism of detection used in the GXP4 Assay Kit.<br />GXP4 activity is measured indirectly. GXP4 converts reduced glutathione (GSH) to oxidized glutathione (GSSG) by reducing hydroperoxide (ROOH). GSSG is then converted back to its reduced form by glutathione reductase (GR) in the presence of NAPDH, which is converted into NAPD⁺. NADPH is intrinsically fluorescent, a property lost upon oxidation to NADP⁺. The decrease in fluorescence (exc λ=340 nm; em λ=450 nm) observed is proportional to the amount of GSSH available for conversion by GR, and to GXP4 activity.

GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) features high binding capacity, specificity, sensitivity, stability, and reusability, making it particularly useful for the affinity purification and immunoprecipitation of DYKDDDDK-tagged fusion proteins expressed in common protein expression systems such as bacteria, yeast, and mammalian cells.