Cell-based Assays

EdU in vivo cell proliferation Kit provides a superior alternative to BrdU and [3H]-thymidine assays for detection of cell proliferation in animals. In principal, EdU (5-ethynyl-2’-deoxyuridine), a thymidine analog, is administered to animals. EdU is incorporated into DNA during DNA synthesis and has a clickable functionality. Detection of cell division in tissues can be performed using microscopy, flow cytometry or in high throughput screening methods. The in vivo kits are thus designed in a sense that we provide additional EdU for the administering step. The three variants of baseclick EdU in vivo kits are: 1. Imaging kit (BCK-EdU-IM100+IV) 2. Flow cytometry kit (BCK-EdU-FC50+IV) 3. High throughput screening – HTS kit (BCK-EdU-HTS2+IV)

EdU in vivo cell proliferation Kit provides a superior alternative to BrdU and [3H]-thymidine assays for detection of cell proliferation in animals. In principal, EdU (5-ethynyl-2’-deoxyuridine), a thymidine analog, is administered to animals. EdU is incorporated into DNA during DNA synthesis and has a clickable functionality. Detection of cell division in tissues can be performed using microscopy, flow cytometry or in high throughput screening methods. The in vivo kits are thus designed in a sense that we provide additional EdU for the administering step. The three variants of baseclick EdU in vivo kits are: 1. Imaging kit (BCK-EdU-IM100+IV) 2. Flow cytometry kit (BCK-EdU-FC50+IV) 3. High throughput screening – HTS kit (BCK-EdU-HTS2+IV)

EdU in vivo cell proliferation Kit provides a superior alternative to BrdU and [3H]-thymidine assays for detection of cell proliferation in animals. In principal, EdU (5-ethynyl-2’-deoxyuridine), a thymidine analog, is administered to animals. EdU is incorporated into DNA during DNA synthesis and has a clickable functionality. Detection of cell division in tissues can be performed using microscopy, flow cytometry or in high throughput screening methods. The in vivo kits are thus designed in a sense that we provide additional EdU for the administering step. The three variants of baseclick EdU in vivo kits are: 1. Imaging kit (BCK-EdU-IM100+IV) 2. Flow cytometry kit (BCK-EdU-FC50+IV) 3. High throughput screening – HTS kit (BCK-EdU-HTS2+IV)

All EdU based proliferation kits on the market are patented by our company since 2007. Using EdU shows several advantages over other cell proliferation assays (BrdU or 3H-thymidine) making it a superior evaluation tool for monitoring genotoxicity, evaluation of anticancer drugs or cell cycle analysis. Measured are only the cells in vitro and/or in vivo that are actively dividing. Healthy cells that are not dividing are not detected and can thus give no false positives. The assay can be used for single cell proliferation analysis as well as cell division in animals. After incorporation of EdU, cell proliferation can be detected in different organs. The amount of EdU used in the assays shows no toxicity in cells as well as in animals thus does not influence the results of experiments. The kits are designed for screening in 96 well-plates. They contain special reagents and a dedicated protocol for read-outs using plate reader. The kits are available for the following wavelenghts: 488 nm

EdU in vivo cell proliferation Kit provides a superior alternative to BrdU and [3H]-thymidine assays for detection of cell proliferation in animals. In principal, EdU (5-ethynyl-2’-deoxyuridine), a thymidine analog, is administered to animals. EdU is incorporated into DNA during DNA synthesis and has a clickable functionality. Detection of cell division in tissues can be performed using microscopy, flow cytometry or in high throughput screening methods. The in vivo kits are thus designed in a sense that we provide additional EdU for the administering step. The three variants of baseclick EdU in vivo kits are: 1. Imaging kit (BCK-EdU-IM100+IV) 2. Flow cytometry kit (BCK-EdU-FC50+IV) 3. High throughput screening – HTS kit (BCK-EdU-HTS2+IV)

EdU in vivo cell proliferation Kit provides a superior alternative to BrdU and [3H]-thymidine assays for detection of cell proliferation in animals. In principal, EdU (5-ethynyl-2’-deoxyuridine), a thymidine analog, is administered to animals. EdU is incorporated into DNA during DNA synthesis and has a clickable functionality. Detection of cell division in tissues can be performed using microscopy, flow cytometry or in high throughput screening methods. The in vivo kits are thus designed in a sense that we provide additional EdU for the administering step. The three variants of baseclick EdU in vivo kits are: 1. Imaging kit (BCK-EdU-IM100+IV) 2. Flow cytometry kit (BCK-EdU-FC50+IV) 3. High throughput screening – HTS kit (BCK-EdU-HTS2+IV)

EdU in vivo cell proliferation Kit provides a superior alternative to BrdU and [3H]-thymidine assays for detection of cell proliferation in animals. In principal, EdU (5-ethynyl-2’-deoxyuridine), a thymidine analog, is administered to animals. EdU is incorporated into DNA during DNA synthesis and has a clickable functionality. Detection of cell division in tissues can be performed using microscopy, flow cytometry or in high throughput screening methods. The in vivo kits are thus designed in a sense that we provide additional EdU for the administering step. The three variants of baseclick EdU in vivo kits are: 1. Imaging kit (BCK-EdU-IM100+IV) 2. Flow cytometry kit (BCK-EdU-FC50+IV) 3. High throughput screening – HTS kit (BCK-EdU-HTS2+IV)

All EdU based proliferation kits on the market are patented by our company since 2007. Using EdU shows several advantages over other cell proliferation assays (BrdU or 3H-thymidine) making it a superior evaluation tool for monitoring genotoxicity, evaluation of anticancer drugs or cell cycle analysis. Measured are only the cells in vitro and/or in vivo that are actively dividing. Healthy cells that are not dividing are not detected and can thus give no false positives. The assay can be used for single cell proliferation analysis as well as cell division in animals. After incorporation of EdU, cell proliferation can be detected in different organs. The amount of EdU used in the assays shows no toxicity in cells as well as in animals thus does not influence the results of experiments. The kits are designed for screening in 96 well-plates. They contain special reagents and a dedicated protocol for read-outs using plate reader. The kits are available for the following wavelenghts: 488 nm

All EdU based proliferation kits on the market are patented by our company since 2007. Using EdU shows several advantages over other cell proliferation assays (BrdU or 3H-thymidine) making it a superior evaluation tool for monitoring genotoxicity, evaluation of anticancer drugs or cell cycle analysis. Measured are only the cells in vitro and/or in vivo that are actively dividing. Healthy cells that are not dividing are not detected and can thus give no false positives. The assay can be used for single cell proliferation analysis as well as cell division in animals. After incorporation of EdU, cell proliferation can be detected in different organs. The amount of EdU used in the assays shows no toxicity in cells as well as in animals thus does not influence the results of experiments. The kits are designed for fluorescence microscopy. They contain special reagents and a dedicated protocol for read-outs using fluorescence microscopy on either cells in suspension or attached cells. The k