Cell-based Assays

The Sensitive EdU Cell Proliferation Kits combine all the advantages of their very popular predecessors – the EdU Cell Proliferation Kits – with a brand new fluorescence enhancer system. Therefore, they are just as easy to handle and as reliable, while showing outstanding efficiency and greatly enhanced signal and sensitivity. Profit from: » Exceptional sensitivity and enhanced fluorescence signals » Lower copper concentrations needed » Better signal-to-noise ratio » Optimized for low content HTS and Flow Cytometry analysis We offer these Sensitive EdU Cell Proliferation Kits for three different ranges of application: cell proliferation analysis by imaging (IM), by flow cytometry (FC) and by high throughput screening analysis (HTS). The IM kits are available for the following wavelenghts: 488 nm and 647 nm The FC kits are available for the following wavelenghts: 488 nm and 647 nm The HTS kits are available for the following wavelenghts: 488 nm and 555 nm

The Sensitive EdU Cell Proliferation Kits combine all the advantages of their very popular predecessors – the EdU Cell Proliferation Kits – with a brand new fluorescence enhancer system. Therefore, they are just as easy to handle and as reliable, while showing outstanding efficiency and greatly enhanced signal and sensitivity. Profit from: » Exceptional sensitivity and enhanced fluorescence signals » Lower copper concentrations needed » Better signal-to-noise ratio » Optimized for low content HTS and Flow Cytometry analysis We offer these Sensitive EdU Cell Proliferation Kits for three different ranges of application: cell proliferation analysis by imaging (IM), by flow cytometry (FC) and by high throughput screening analysis (HTS). The IM kits are available for the following wavelenghts: 488 nm and 647 nm The FC kits are available for the following wavelenghts: 488 nm and 647 nm The HTS kits are available for the following wavelenghts: 488 nm and 555 nm

The Sensitive EdU Cell Proliferation Kits combine all the advantages of their very popular predecessors – the EdU Cell Proliferation Kits – with a brand new fluorescence enhancer system. Therefore, they are just as easy to handle and as reliable, while showing outstanding efficiency and greatly enhanced signal and sensitivity. Profit from: » Exceptional sensitivity and enhanced fluorescence signals » Lower copper concentrations needed » Better signal-to-noise ratio » Optimized for low content HTS and Flow Cytometry analysis We offer these Sensitive EdU Cell Proliferation Kits for three different ranges of application: cell proliferation analysis by imaging (IM), by flow cytometry (FC) and by high throughput screening analysis (HTS). The IM kits are available for the following wavelenghts: 488 nm and 647 nm The FC kits are available for the following wavelenghts: 488 nm and 647 nm The HTS kits are available for the following wavelenghts: 488 nm and 555 nm

The Sensitive EdU Cell Proliferation Kits combine all the advantages of their very popular predecessors – the EdU Cell Proliferation Kits – with a brand new fluorescence enhancer system. Therefore, they are just as easy to handle and as reliable, while showing outstanding efficiency and greatly enhanced signal and sensitivity. Profit from: » Exceptional sensitivity and enhanced fluorescence signals » Lower copper concentrations needed » Better signal-to-noise ratio » Optimized for low content HTS and Flow Cytometry analysis We offer these Sensitive EdU Cell Proliferation Kits for three different ranges of application: cell proliferation analysis by imaging (IM), by flow cytometry (FC) and by high throughput screening analysis (HTS). The IM kits are available for the following wavelenghts: 488 nm and 647 nm The FC kits are available for the following wavelenghts: 488 nm and 647 nm The HTS kits are available for the following wavelenghts: 488 nm and 555 nm

The Sensitive EdU Cell Proliferation Kits combine all the advantages of their very popular predecessors – the EdU Cell Proliferation Kits – with a brand new fluorescence enhancer system. Therefore, they are just as easy to handle and as reliable, while showing outstanding efficiency and greatly enhanced signal and sensitivity. Profit from: » Exceptional sensitivity and enhanced fluorescence signals » Lower copper concentrations needed » Better signal-to-noise ratio » Optimized for low content HTS and Flow Cytometry analysis We offer these Sensitive EdU Cell Proliferation Kits for three different ranges of application: cell proliferation analysis by imaging (IM), by flow cytometry (FC) and by high throughput screening analysis (HTS). The IM kits are available for the following wavelenghts: 488 nm and 647 nm The FC kits are available for the following wavelenghts: 488 nm and 647 nm The HTS kits are available for the following wavelenghts: 488 nm and 555 nm

The Sensitive EdU Cell Proliferation Kits combine all the advantages of their very popular predecessors – the EdU Cell Proliferation Kits – with a brand new fluorescence enhancer system. Therefore, they are just as easy to handle and as reliable, while showing outstanding efficiency and greatly enhanced signal and sensitivity. Profit from: » Exceptional sensitivity and enhanced fluorescence signals » Lower copper concentrations needed » Better signal-to-noise ratio » Optimized for low content HTS and Flow Cytometry analysis We offer these Sensitive EdU Cell Proliferation Kits for three different ranges of application: cell proliferation analysis by imaging (IM), by flow cytometry (FC) and by high throughput screening analysis (HTS). The IM kits are available for the following wavelenghts: 488 nm and 647 nm The FC kits are available for the following wavelenghts: 488 nm and 647 nm The HTS kits are available for the following wavelenghts: 488 nm and 555 nm

The Sensitive EdU Cell Proliferation Kits combine all the advantages of their very popular predecessors – the EdU Cell Proliferation Kits – with a brand new fluorescence enhancer system. Therefore, they are just as easy to handle and as reliable, while showing outstanding efficiency and greatly enhanced signal and sensitivity. Profit from: » Exceptional sensitivity and enhanced fluorescence signals » Lower copper concentrations needed » Better signal-to-noise ratio » Optimized for low content HTS and Flow Cytometry analysis We offer these Sensitive EdU Cell Proliferation Kits for three different ranges of application: cell proliferation analysis by imaging (IM), by flow cytometry (FC) and by high throughput screening analysis (HTS). The IM kits are available for the following wavelenghts: 488 nm and 647 nm The FC kits are available for the following wavelenghts: 488 nm and 647 nm The HTS kits are available for the following wavelenghts: 488 nm and 555 nm

baseclick’s ClickTech Library Kit full-length mRNA_Seq V2.0 has been developed as a method for sequencing total mRNA of cells for transcriptome analysis. The kit provides reagents for cDNA synthesis from any RNA pool and introduces a single azide nucleotide at the 3’End of cDNA. This azido-modified cDNA can react with an 5’-alkyne-modified adapter sequence in a highly specific and biorthogonal manner under benign click reaction conditions. With the included specially designed PCR Primers, the 3’-adapter-clicked cDNA can be amplified via PCR, enabling subsequent NGS experiments or alternatively targeted gene amplifications

All EdU based proliferation kits on the market are patented by our company. This click assay has been optimized for the detection of T cell proliferation. Of utmost importance in immunology research is the activation of T cells. This is normally done by BrdU assays and in special applications by fluorescent cell tracing reagents. The used BrdU assays have several limitations as it is time consuming (4 h) and it requires harsh, denaturing conditions of tissue to allow anti-BrdU antibodies to reach the genomic DNA. Additionally the low sensitivity requires a high number of target cells. The indirect fluorescent cell tracing reagent method depends on long incubation time (4 – 7 days) and in higher concentration shows cytotoxicity. The EdU assay is quicker (1 h), simpler, and highly sensitive by the bio orthogonal CuAAC reaction. Therefore, EdU is a superior sensitive alternative to BrdU and the dilution methods, is less cytotoxic and allows improved detection of e.g. interferon gamma r