Cell-based Assays

The SLP-HS Single Reagent Set II is able to detect both peptidoglycan (PG) and (1->3)-β-D-glucan (BDG). The product uses a self-defense mechanism of silkworm Bombyx mori which leads to a color change by building a black melanin pigment in the presence of microbial contamination. Since PG is found in all bacterial cell walls and BDG in most fungi, the SLP (silkworm larvae plasma)-reagent constitutes a highly sensitive and broadband method for detection of microbial contamination

PYROGENS & ENDOTOXIN DETECTION AT FUJIFIM PYROGENS ARE FEVER-INDUCING SUBSTANCES THAT IF ADMINISTERED TO HUMANS ABOVE CERTAIN CONCENTRATIONS MAY CAUSE SEVERE INFLAMMATION AND IN SOME CASES EVEN LEAD TO DEATH. WHILE ENDOGENOUS PYROGENS MAINLY CONSIST OF CYTOKINES, EXOGENOUS PYROGENS HAVE SIGNIFICANTLY GREATER STRUCTURAL DIVERSITY, COMPRISING A BROAD RANGE OF DIFFERENT MOLECULES SUCH AS LIPOPOLYSACCHARIDES, GLUCANES OR SMALL ORGANIC COMPOUNDS SUCH AS 2,4-DINITROPHENOL. AMONG EXOGENOUS PYROGENS THE GRAM-NEGATIVE CELL-WALL COMPONENT LIPOPOLYSACCHARIDE (LPS) IS GENERALLY CONSIDERED AS BIOLOGICALLY MOST ACTIVE. LPS CONSIST OF LARGE GLYCOCONJUGATES, INCLUDING A LIPIDIC MOIETY AND A POLYSACCHARIDE CORE TERMED “O-ANTIGEN”. DUE TO THEIR TOXIC PROPERTIES, THEY ARE USUALLY REFERRED TO AS ENDOTOXINS. WHEN ENTERING THE BLOODSTREAM, ENDOTOXINS QUICKLY INTERACT WITH IMMUNE CELLS AND INDUCE STRONG INFLAMMATORY REACTION, RESULTING IN AN INCREASE OF THE BODY TEMPERATURE. OWING TO THEIR GREAT HEAT RESI

PYROGENS & ENDOTOXIN DETECTION AT FUJIFIM PYROGENS ARE FEVER-INDUCING SUBSTANCES THAT IF ADMINISTERED TO HUMANS ABOVE CERTAIN CONCENTRATIONS MAY CAUSE SEVERE INFLAMMATION AND IN SOME CASES EVEN LEAD TO DEATH. WHILE ENDOGENOUS PYROGENS MAINLY CONSIST OF CYTOKINES, EXOGENOUS PYROGENS HAVE SIGNIFICANTLY GREATER STRUCTURAL DIVERSITY, COMPRISING A BROAD RANGE OF DIFFERENT MOLECULES SUCH AS LIPOPOLYSACCHARIDES, GLUCANES OR SMALL ORGANIC COMPOUNDS SUCH AS 2,4-DINITROPHENOL. AMONG EXOGENOUS PYROGENS THE GRAM-NEGATIVE CELL-WALL COMPONENT LIPOPOLYSACCHARIDE (LPS) IS GENERALLY CONSIDERED AS BIOLOGICALLY MOST ACTIVE. LPS CONSIST OF LARGE GLYCOCONJUGATES, INCLUDING A LIPIDIC MOIETY AND A POLYSACCHARIDE CORE TERMED “O-ANTIGEN”. DUE TO THEIR TOXIC PROPERTIES, THEY ARE USUALLY REFERRED TO AS ENDOTOXINS. WHEN ENTERING THE BLOODSTREAM, ENDOTOXINS QUICKLY INTERACT WITH IMMUNE CELLS AND INDUCE STRONG INFLAMMATORY REACTION, RESULTING IN AN INCREASE OF THE BODY TEMPERATURE. OWING TO THEIR GREAT HEAT RESI

PYROGENS & ENDOTOXIN DETECTION AT FUJIFIM PYROGENS ARE FEVER-INDUCING SUBSTANCES THAT IF ADMINISTERED TO HUMANS ABOVE CERTAIN CONCENTRATIONS MAY CAUSE SEVERE INFLAMMATION AND IN SOME CASES EVEN LEAD TO DEATH. WHILE ENDOGENOUS PYROGENS MAINLY CONSIST OF CYTOKINES, EXOGENOUS PYROGENS HAVE SIGNIFICANTLY GREATER STRUCTURAL DIVERSITY, COMPRISING A BROAD RANGE OF DIFFERENT MOLECULES SUCH AS LIPOPOLYSACCHARIDES, GLUCANES OR SMALL ORGANIC COMPOUNDS SUCH AS 2,4-DINITROPHENOL. AMONG EXOGENOUS PYROGENS THE GRAM-NEGATIVE CELL-WALL COMPONENT LIPOPOLYSACCHARIDE (LPS) IS GENERALLY CONSIDERED AS BIOLOGICALLY MOST ACTIVE. LPS CONSIST OF LARGE GLYCOCONJUGATES, INCLUDING A LIPIDIC MOIETY AND A POLYSACCHARIDE CORE TERMED “O-ANTIGEN”. DUE TO THEIR TOXIC PROPERTIES, THEY ARE USUALLY REFERRED TO AS ENDOTOXINS. WHEN ENTERING THE BLOODSTREAM, ENDOTOXINS QUICKLY INTERACT WITH IMMUNE CELLS AND INDUCE STRONG INFLAMMATORY REACTION, RESULTING IN AN INCREASE OF THE BODY TEMPERATURE. OWING TO THEIR GREAT HEAT RESI

THE LIMULUS PS SINGLE TEST IS A KIT COMPOSED OF LAL ENDOTOXIN SPECIFIC REAGENT AND AN AFFINITY RESIN SUSPENSION KNOWN AS PYROSEP™, DESIGNED TO OVERCOME ANY PRODUCT INTERFERENCE BY ADSORBING POTENTIAL ENDOTOXIN IN SAMPLES WHILE WASHING AWAY THE INHIBITORY COMPONENTS. PRODUCT FEATURES: • ENDOTOXIN-SPECIFIC LYSATE, AVOIDS FALSE POSITIVE RESULTS FROM GLUCANS • KTA ASSAY IS PERFORMED IN TUBE READER • ALLOWS ENDOTOXIN SPECIFIC MEASUREMENT OF SAMPLES WHICH INHIBIT TRADITIONAL LAL ASSAYS • FAT-SOLUBLE VITAMINS, OILS AND FATS CAN BE TESTED IF THEY CAN BE DISSOLVED IN ETHANOL.

THE CONTROL STANDARD ENDOTOXIN IS A LYOPHILIZED PRODUCT COMPOSED OF 500 NG/VIAL OF ENDOTOXIN, PURIFIED FROM E. COLI UKT-B STRAIN, THAT SERVES AS AN ACCURATE AND DEPENDABLE STANDARD FOR ENDOTOXIN TESTING.

THE LPS FOR ENDOTOXIN INDICATOR IS COMPOSED OF E. COLI O55:B5 (>100,000 EU/VIAL) AND SERVES AS A STOCK SOLUTION TO PREPARE MULTIPLE ENDOTOXIN INDICATORS FOR DEPYROGENATION VALIDATION STUDIES. THE PREPARED ENDOTOXIN INDICATORS MAY BE TESTED USING REAGENTS FROM OUR PYROSTAR™ ES-F SERIES. KEY FEATURES E. COLI 055:B5 (> 100,000 EU/VIAL) CAN MAKE MULTIPLE ENDOTOXIN INDICATORS FROM ONE VIAL USED FOR DRY HEAT OVEN VALIDATION STUDIES

THE PYROSTAR™ ES-F SERIES OF REAGENTS REPRESENT OUR UNIQUELY ENDOTOXIN SPECIFIC LINE OF LIMULUS AMEBOCYTE LYSATE PRODUCTS, WHICH ARE FORMULATED TO BE USED EITHER QUALITATIVELY AS A GEL-CLOT REAGENT OR QUANTITATIVELY AS A KINETIC-TURBIDIMETRIC REAGENT. PRODUCT FEATURES : • ENDOTOXIN-SPECIFIC LYSATE, AVOIDS FALSE POSITIVE RESULTS FROM GLUCANS • AVAILABLE IN MULTI-TESTS VIALS OR SINGLE-TEST VIALS • CAN BE USED AS EITHER A GEL-CLOT OR KINETIC-TURBIDIMETRIC ASSAY (KTA) REAGENT • (KTA) ASSAYS CAN BE PERFORMED IN TUBE READER OR MICROPLATE READER • GEL-CLOT LYSATE SENSITIVITIES RANGE FROM 0.015 TO 0.25 EU/ML • AVAILABLE WITH MATCHED CONTROL STANDARD ENDOTOXIN (CSE) • PYROSTAR™ ES-F REAGENTS ARE AVAILABLE WITH A KTA QUANTITATIVE RANGE OF EITHER 0.001 EU/ML TO 10 EU/ML. THE KTA QUANTITATIVE RANGE IS RELATED TO THE GEL-CLOT SENSITIVITY. • 100 UL SAMPLE SIZE WHEN USED WITH TUBE READER; 50 UL SAMPLE SIZE WHEN USED WITH MICROPLATE READER

THE PYROSTAR™ ES-F SERIES OF REAGENTS REPRESENT OUR UNIQUELY ENDOTOXIN SPECIFIC LINE OF LIMULUS AMEBOCYTE LYSATE PRODUCTS, WHICH ARE FORMULATED TO BE USED EITHER QUALITATIVELY AS A GEL-CLOT REAGENT OR QUANTITATIVELY AS A KINETIC-TURBIDIMETRIC REAGENT. PRODUCT FEATURES : • ENDOTOXIN-SPECIFIC LYSATE, AVOIDS FALSE POSITIVE RESULTS FROM GLUCANS • AVAILABLE IN MULTI-TESTS VIALS OR SINGLE-TEST VIALS • CAN BE USED AS EITHER A GEL-CLOT OR KINETIC-TURBIDIMETRIC ASSAY (KTA) REAGENT • (KTA) ASSAYS CAN BE PERFORMED IN TUBE READER OR MICROPLATE READER • GEL-CLOT LYSATE SENSITIVITIES RANGE FROM 0.015 TO 0.25 EU/ML • AVAILABLE WITH MATCHED CONTROL STANDARD ENDOTOXIN (CSE) • PYROSTAR™ ES-F REAGENTS ARE AVAILABLE WITH A KTA QUANTITATIVE RANGE OF EITHER 0.001 EU/ML TO 10 EU/ML. THE KTA QUANTITATIVE RANGE IS RELATED TO THE GEL-CLOT SENSITIVITY. • 100 UL SAMPLE SIZE WHEN USED WITH TUBE READER; 50 UL SAMPLE SIZE WHEN USED WITH MICROPLATE READER