Cell Line

The HLA-C*08:02 K562 Cell Line is an engineered human lymphoblast K562 cell line expressing human HLA-C*08:02 driven by an EF1a promoter. This cell line was generated by transduction with HLA-C*08:02 Lentivirus (BPS Bioscience #78930). This cell line has been validated by flow cytometry and co-culture assays with Jurkat cells expressing KRAS G12D, in the presence of wild type and mutant KRAS peptides

MAGE-A4 CD8⁺ NFAT-Luciferase Reporter Jurkat Cell Line was generated from T Cell Receptor (TCR) Knockout NFAT Luciferase Reporter Jurkat Cell Line (BPS Bioscience #78556</a>) by overexpression of human CD8 (NM_001768.6) and a MAGE-A4 (Melanoma-associated antigen 4)-directed TCR using lentiviral transduction (CD8a Lentivirus #78648 and MAGE-A4-Specific TCR Lentivirus #78935). This human MAGE-A4 TCR specifically recognizes an antigen corresponding to MAGE-A4 peptide, amino acids 230-239 (GVYDGREHTV).

The Untransduced T Cells (NY-ESO-1 TCR-T Negative Control) were produced by mock lentiviral transduction of human primary CD4⁺ and CD8⁺T cells. These cells are subjected to comparable manipulations as TCR (T cell receptor)-T cells: activation, spinoculation (without lentivirus), and antigen specific stimulation. These T cells are designed as negative controls in experiments using lentivirus-transduced TCR-T cells, such as NY-ESO-1 (c259) TCR-T Cells (BPS Bioscience #78990</a>).

The NY-ESO-1 (c259) TCR-T Cells are generated by high-titer lentiviral transduction of human primary CD4⁺ and CD8⁺ T cells <u>with NY-ESO-1-Specific TCR Lentivirus (Clone c259) (#78676)</u>. These ready-to-use TCR (T cell receptor) -T cells express the human TCR clone c259, that specifically recognizes the antigen NY-ESO-1 (New York esophageal squamous cell carcinoma 1). These TCR-T cells have been validated by flow cytometry (to determine the TCR expression) and co-culture assays (IFN-γ staining and degranulation).

IL-6 Responsive Luciferase Reporter HEK293 Cell Line is a HEK293 cell line designed to monitor the IL-6 (interleukin 6)/IL-6 receptor interaction. These cells express endogenous IL-6 receptor (IL-6R and gp130) and were engineered to express firefly luciferase under the control of a STAT response element. This cell line has been validated to respond to human interleukin-6 (IL-6), IL-27, and further functional validation experiments showed that IL-6-induced luciferase activity was decreased by the JAK inhibitor CP 690,550 and by an anti-IL-6R neutralizing antibody.

eGFP Daudi Cell Line is a Daudi cell line engineered to express enhanced GFP (eGFP) driven by an EF1a promoter. This cell line was generated by transduction with Enhanced GFP Lentivirus (Puromycin) (BPS Bioscience #78639</a>). This cell line has been validated by flow cytometry for eGFP expression.

HER3 CHO Cell Line is a CHO-K1 cell line expressing HER3 (human epidermal growth factor receptor 3) (NM_001982.2, also known as receptor tyrosine-protein kinase erbB-3) under the control of a cytomegalovirus (CMV) promoter. This cell line was generated by lipid-mediated transfection followed by hygromycin selection and limited dilution. Individual clones were screened for HER3 expression levels by flow cytometry, and a clone was selected to generate this cell line.

The TSLP Responsive Luciferase Reporter Ba/F3 Cell Line is a murine Ba/F3 cell line engineered to express both TSLP-R (thymic stromal lymphopoietin receptor, also known as CRLF2, cytokine receptor like factor 2) (NM_022148.4) and IL-7Ra (Interleukin 7 receptor alpha) (NM_002185.5) separated by a self-cleaving P2A peptide. The construct was delivered bytransduction of STAT5 Luciferase Reporter Ba/F3 cells (#79772</a>), which express a firefly luciferase reporter driven by STAT5 response elements located upstream of the minimal TATA promoter. After activation by TSLP, the endogenous transcription factor STAT5 binds to the response elements, inducing transcription of the luciferase reporter gene. This cell line has been validated to respond to TSLP. Additional functional validation demonstrates that TSLP-induced luciferase activity can be inhibited by either anti-TSLP or anti-TSLPR neutralizing antibodies. TSLP binds to TSLPR, recruits the IL-7Ra co-receptor and activates downstream JAK1/

eGFP/Firefly Luciferase THP-1 Cell Line is a THP-1 cell line expressing firefly luciferase and enhanced GFP (eGFP) driven by an EF1a promoter. The cells were generated by transduction with Firefly Luciferase-eGFP lentivirus (BPS Bioscience #78741), which is a SIN (self-inactivating) lentivirus. Please note this product may be subject to fees, we invite you to contact your local office.