ELISA Reagents

8 ml of Stop Solution, containing 0.2 M sulfuric acid

12 ml of 3,3',5,5'-tetramethylbenzidine (TMB) in buffered solution

MagicBrite ECL PicoPlus Substrate Kit is a sensitive, enhanced luminol-based chemiluminescent substrate (ECL) for the detection of horseradish peroxidase (HRP) in immunoblotting and other immunoassay applications. The ECL substrate detects low pico gram amounts of antigen with stable and long light output (up to 4 hours), which can be visualized using X-ray films or CCD-based imagers. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.<br /><br>Features:<br /><strong>Sensitivity:</strong> low picogram<br /> <strong>Stability:</strong> 1-hr working solution stability, 1-year kit stability at room temperature. <br /><strong>Compatibility:</strong> nitrocellulose and PVDF membranes<br /> <strong>Signal duration:</strong> up to 4 hours<br /> <strong>Recommended dilution:</strong> primary antibody: 1:1,000 1:5,000; secondary antibody: 1:20,000 to 1:100,000

MagicBrite ECL PicoPlus Substrate Kit is a sensitive, enhanced luminol-based chemiluminescent substrate (ECL) for the detection of horseradish peroxidase (HRP) in immunoblotting and other immunoassay applications. The ECL substrate detects low pico gram amounts of antigen with stable and long light output (up to 4 hours), which can be visualized using X-ray films or CCD-based imagers. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.<br /><br>Features:<br /><strong>Sensitivity:</strong> low picogram<br /> <strong>Stability:</strong> 1-hr working solution stability, 1-year kit stability at room temperature. <br /> <strong>Compatibility:</strong> nitrocellulose and PVDF membranes<br /> <strong>Signal duration:</strong> up to 4 hours<br /> <strong>Recommended dilution:</strong> primary antibody: 1:1,000 1:5,000; secondary antibody: 1:20,000 to 1:100,000

MagicBrite ECL PicoPlus Substrate Kit is a sensitive, enhanced luminol-based chemiluminescent substrate (ECL) for the detection of horseradish peroxidase (HRP) in immunoblotting and other immunoassay applications. The ECL substrate detects low pico gram amounts of antigen with stable and long light output (up to 4 hours), which can be visualized using X-ray films or CCD-based imagers. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.<br /><br>Features:<br /><strong>Sensitivity:</strong> low picogram<br /> <strong>Stability:</strong> 1-hr working solution stability, 1-year kit stability at room temperature. <br /> <strong>Compatibility:</strong> nitrocellulose and PVDF membranes<br /> <strong>Signal duration:</strong> up to 4 hours<br /> <strong>Recommended dilution:</strong> primary antibody: 1:1,000 1:5,000; secondary antibody: 1:20,000 to 1:100,000

MagicBrite ECL DuraPlus Substrate Kit is a sensitive, enhanced luminol-based chemiluminescent substrate (ECL) for the detection of horseradish peroxidase (HRP) in immunoblotting and other immunoassay applications. The ECL substrate detects high femo gram amounts of antigen with stable and long light output (5 to 24 hours), which can be visualized using X-ray films or CCD-based imagers. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.<br /> <br /> Features:<br /> <strong>Sensitivity:</strong> high femo gram<br /> <strong>Stability:</strong> 1-hr working solution stability, 1-year kit stability at room temperature.<br /> <strong>Compatibility:</strong> nitrocellulose and PVDF membranes<br /> <strong>Signal duration:</strong> 5 to 24 hours<br /> <strong>Recommended dilution:</strong> primary antibody: 1:1,000 1:20,000; secondary antibody: 1:20,000 to 1:200,000</p>

MagicBrite ECL DuraPlus Substrate Kit is a sensitive, enhanced luminol-based chemiluminescent substrate (ECL) for the detection of horseradish peroxidase (HRP) in immunoblotting and other immunoassay applications. The ECL substrate detects high femo gram amounts of antigen with stable and long light output (5 to 24 hours), which can be visualized using X-ray films or CCD-based imagers. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration. <br /> <br /> Features:<br /> <strong>Sensitivity:</strong> high femo gram<br /> <strong>Stability:</strong> 1-hr working solution stability, 1-year kit stability at room temperature. <br /> <strong>Compatibility:</strong> nitrocellulose and PVDF membranes<br /> <strong>Signal duration:</strong> 5 to 24 hours<br /><strong>Recommended dilution:</strong> primary antibody: 1:1,000 1:20,000; secondary antibody: 1:20,000 to 1:200,000

The FineTest Lysis Buffer produced is a traditional rapid lyse solution for tissue cells.Protein samples obtained by Finetest Lysis Buffer can be used for ELISA. This product can be used on animal, plant cell or tissue samples. The main components are 50mM Tris(pH7.2- 7.4), 0.9% NaCl, 0.1% SDS. When using this lysate, the user can add specific inhibitors or not according to the specific purpose. Protein samples obtained by FineTest Lysis Buffer can use FineTest BCA protein assay kit (K001) to measure protein concentration.Due to the high concentration of detergent, the protein concentration of the sample obtained from the FineTest Lysis Buffer cannot be determined by Bradford method.