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DMEM/F-12 Medium (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a 1:1 mixture of DMEM medium and Ham's F-12 medium, based on DMEM medium. F-12 was added to enrich the nutrient content, containing a variety of trace elements. Dulbecco Modified Eagle Medium (DMEM) is a modified Eagle Basic Medium (BME) with four times the amino acid and vitamin concentration of BME. Ham's F-12, based on Ham's F-10 medium, significantly increased the concentration of choline, inositol, putputylamine and several amino acids. DMEM/F-12 medium is widely used to support the growth of a variety of mammalian cells, including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium, and it is also suitable for the culture of mammalian cells with low serum content and clone density. DMEM/F12 Medium contains amino acids, vitamins, inorganic salts and other components required for