Histology & Microscopy

Amylo-Glo RTD Ready to Dilute Staining reagent is designed to stain amyloid plaques in tissue sections. This novel marker has several advantages over other conventional markers such as Thioflavin S and Congo Red because of its unique chemical and spectral properties. (L. Schmued et al. (2012) J.Neuroscience Methods 209:120- 126). Using Amylo-Glo results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. Its brightness makes it ideal for low magnification quantification studies, while its unique excitation/emission profile and mild staining conditions makes it ideal for combination for multiple immunofluorescent labeling studies. Amylo-Glo RTD is compatible with fresh, frozen, and formalin-fixed immunohistochemistry or cytochemistry, and it is particularly good for confocal and multiple labeling because of its high fluorescent intensity and high resistance to photo-bleaching. Moreover because Amylo-Glo

Pan Blocking Kit contains FcR Blocking Reagent and Accessory Blocker for specific and non-specific blocking.

Pre-treatment is an essential step for effective FISH on Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections. This kit contains key reagents used to pretreat FFPE tissue sections from dewaxing with xylene through proteolytic digestion to help permeabilization of cell membranes to facilitate penetration of fluorescence labeled probes. After proteolytic digestion, proceed with subsequent steps with the FISH assay protocol.

Pre-treatment is an essential step for effective FISH on Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections. This kit contains key reagents used to pretreat FFPE tissue sections from dewaxing with xylene through proteolytic digestion to help permeabilization of cell membranes to facilitate penetration of fluorescence labeled probes. After proteolytic digestion, proceed with subsequent steps with the FISH assay protocol.

Pre-treatment is an essential step for effective FISH on Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections. This kit contains key reagents used to pretreat FFPE tissue sections from dewaxing with xylene through proteolytic digestion to help permeabilization of cell membranes to facilitate penetration of fluorescence labeled probes. After proteolytic digestion, proceed with subsequent steps with the FISH assay protocol.

Protein Staining Solution stains proteins in polyacrylamide gel after SDS gel electrophoresis (detection limit >= 8 ng of protein) with *CoomassieR-G250. All processes including washing and destaining processes can be performed in 1.5 hr. After staining of gel (20 min), proteins can be seen before destaining. Overnight staining and overnight destaining is allowable.

DAPI is the recommended counterstain for FISH probes. This concentration of DAPI provides a weaker DAPI stain usually preferred with smaller probe signals such as FISH probes.