Histology & Microscopy

WB Lysis Buffer

SuperKine™ Enhanced Antifade Mounting Medium with DAPI is compatible with a wide range of fluorescent dyes and protects the entire visible and infrared spectrum from fluorescence signal quenching.

DAPI (4',6-diamidino-2-phenylindole) is a fluorescent dye which can bind DNA strands robustly, the fluorescence being detected by fluorescence microscope. DAPI can dye both live and fixed cells as it can cross intact membrane, with higher efficiency in fixed cells. The molecular formula is C16H15N5·2HCl with 350.25 g/mol molecular weight, CAS Number 28718-90-3. DAPI could pass through the cell and nucleic membranes and bind the double-strand DNA in the nucleus, producing 20 times stronger fluorescence than itself. The efficiency detected by fluorescence microscope is very high (almost 100%), having no side effects for the live cells. The sensitivity for double stranded DNA DAPI staining is many times larger comparing to ethidium bromide (EB). DAPI staining is usually used in cell death detection, as it enters more effectively and generates stronger fluorescence in dead cells. After staining with DAPI, detect with fluorescence microscope or flow cytometry. Blue fluorescent cell would be

Microscope slides coated with 100-nm Aluminum

AP-Red Solution Set is intended to be used as a substrate for an AP-conjugated antibody in chromogenic <i>in situ</i> hybridization (CISH) applications.

HRP-Green Solution Set is intended to be used as a substrate for a HRP-conjugated antibody in chromogenic <i>in situ</i> hybridization (CISH) applications.

The DAB Solution Set is intended to be used as a substrate for a HRP-conjugated antibody on formalin-fixed, paraffin-embedded specimens by CISH.

The Blocking Solution is intended to be used for blocking steps in CISH on formalin-fixed, paraffin-embedded specimens.