PEG

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DOPE-PEG-Fluor 555, MW 5,000 is a PEG lipid featuring a DOPE phospholipid and a Fluor 555 dye. DOPE is an unsaturated phospholipid that features prominently in the development of lipid nanoparticles while Fluor 555 is a bright orange cyanine dye that can be used in fluorescence microscopy, FRET and other in vivo imaging techniques.

N-(Amino-PEG4)-N-bis(PEG4-propargyl) HCl salt is a trifunctional linker with two terminal alkynes and a primary amine. The terminal alkynes can be linked to azides using copper click chemistry while the primary amine is reactive towards carboxylic acids, NHS esters, ketones and aldehydes to form a variety of stable bonds.

NH-bis(PEG8-Acid) is a bifunctional reagent containing two carboxylic acids linked together by a secondary amine. The carboxylic acids are open for coupling with amines and alcohols to form amides and esters respectively while the secondary amine can be used as a nucleophile with ketones, aldehydes, and carboxylic acids. The PEG spacers make this molecule water-soluble, potentially altering its DMPK properties.

NH-bis(PEG8-OH) is a homobifunctional reagent containing two alcohols joined together by a secondary amine. The alcohols can be used in a variety of ways such as in forming esters with carboxylic acids, while the secondary amine can be used as a nucleophile in linking with ketones, aldehydes, and carboxylic acids. The PEG spacers make this molecule water-soluble, potentially altering its DMPK properties.

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Benzyl-PEG-Succinimidyl Carbonate, MW 2,000 is a PEG linker containing a succinimidyl carbonate and a benzyl-protected alcohol. The succinimidyl carbonate is a good leaving group that is easily substituted by amines or alcohols with ease under mild conditions. The benzyl protecting group can be removed by hydrogenation to reveal an alcohol group, which is a versatile scaffold towards further building chemical complexity.

DBCO-N-bis(PEG8-acid) is a trifunctional molecule containing two carboxylic acids and a DBCO functional group. The DBCO is reactive towards azides in copper-free click chemistry while the carboxylic acids can be reacted with primary amines in the presence of a coupling reagent to form stable amide bonds. PEG linkers have higher aqueous solubility than their alkane counterparts, which may impact DMPK and ADME in vivo.