Pseudovirus

The bald lentiviral pseudovirion was produced without envelope glycoproteins such as VSV-G or SARS-CoV-2 spike. It contains the firefly luciferase gene driven by a CMV promoter as the reporter. The bald lentiviral pseudovirion can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors.

The bald lentiviral pseudovirion was produced without envelope glycoproteins such as VSV-G or SARS-CoV-2 spike. It contains the eGFP gene driven by a CMV promoter as the reporter. The bald lentiviral pseudovirion can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors.

The bald lentiviral pseudovirion was produced without envelope glycoproteins such as VSV-G or SARS-CoV-2 spike. It contains a firefly luciferase and eGFP cassette (Luc-P2A-eGFP) as the reporters, driven by a CMV promoter. The bald lentiviral pseudovirion can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors.

SARS-CoV-2 virus-like particles are composed of four structural proteins: Membrane protein (M), Nucleocapsid protein (N), Spike protein (S) and Envelope protein (E). VLPs are produced in HEK293 cells.

D1 Dopamine receptor packaged in BacMam for expression in mammalian cells. BacMam provides even, near 100% transduction efficiency in HEK293 cells as well as expression in many other cell lines, iPSCs, and primary cultures. Expression is easily titrateable to your experimental needs or to adjust assay sensitivity. Co-express with Montana Molecular sensors for cAMP signaling, arrestin signaling, and more. This kit includes the D1R BacMam and 500mM sodium butyrate in H2O.

1 mL of alphapseudovirus (Luc) particles, Luciferase reporter

This pseudovirus is a BSL-1 baculovirus vector pseudotyped with Spike proteins, in this case the Spike protein originally sequenced from isolates obtained in Wuhan. Biologists and drug discovery teams can use this reagent to work on COVID-19 projects outside of BSL-3 facilities with standard safety procedures. Once inside the cell, the pseudovirus does not replicate, but delivers a genetically-encoded, fluorescent reporter that expresses bright green fluorescence in the host cell nucleus. If needed, cellular entry can be facilitated with ACE2 and other proteins packaged in BacMam and available from Montana Molecular. 5mL and 60mL sizes are also available. This kit includes the pseudovirus and 500mM sodium butyrate in H2O.

SARS-CoV-2 S protein expression vectors

This pseudovirus is a BSL-1 baculovirus vector pseudotyped with Spike proteins, in this case the Spike protein originally sequenced from isolates obtained in Wuhan. Biologists and drug discovery teams can use this reagent to work on COVID-19 projects outside of BSL-3 facilities with standard safety procedures. Once inside the cell, the pseudovirus does not replicate, but delivers a genetically-encoded, fluorescent reporter that expresses bright green fluorescence in the host cell nucleus. If needed, cellular entry can be facilitated with ACE2 and other proteins packaged in BacMam and available from Montana Molecular. 30mL and 60mL sizes are also available. This kit includes the pseudovirus and 500mM sodium butyrate in H2O.