Secondary Antibodies

Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.

EBS-huA recognizes the third constant domain (CH3) of the alpha chain of human IgA and reacts with both IgA1 and IgA2 isotypes and not with other types of immunoglobulins. IgA type antibodies are secreted by B-cells associated with mucosal epithelia and therefore indicate malignancy if found in lymphoid infiltrates at other locations. Detection of IgA antibodies to EBV in serum may indicate nasopharyngeal carcinoma. Rising IgA anti EBV levels are associated with progression of disease.

SC-05 reacts with a reduction resistant epitope on 80 kDa human secretory component (both free and bound to SIgA). Secretory component is differentially expressed in epithelium, thus SC-05 can identify subpopulations of epithelial cells and epithelial differentiation. Secretory component negative cell lines are not stained with SC-05.

EBS-huG reacts specifically with human IgG and not with other types of immunoglobulins.

EBS-huM reacts specifically with human IgM and not with other types of immunoglobulins

Solution containing antibody 1 mg/ml in buffer (0.05 M sodium phosphate, 0.1 M NaCl, 50% glycerol,pH 7.5). Human immunoglobulin IgG. No reactivity against human IgA, IgM, other serum proteins.

Solution 1.2 mg/ml in the buffer (0.05 M sodium phosphate pH 7.5, 0.1 M NaCl, 50% glycerol, 5 mg/ml BSA). Human immunoglobulin IgG. No cross reactivity to human IgM, IgA or other human plasma proteins

Solution 1.2 mg/ml in the buffer (0.1 M sodium chloride, 0.01M sodium phosphate pH 7.5, 50% glycerol, 5 mg/ml BSA). Human immunoglobulin IgG. No reactivity against human IgA, IgM, other serum proteins

Solution 1.2 mg/ml in the buffer (0.1 M sodium chloride, 0.01M sodium phosphate pH 7.5, 50% glycerol, 5 mg/ml BSA). Human immunoglobulin IgM. No cross-reactivity to human IgG, IgA and other human plasma proteins