Proteins & Peptides

Human USP15 recombinant protein, with N-terminal SUMO- and His-tags, expressed in E. coli.

Human USP21 recombinant protein (Genbank Accession No. Q9UK80), with N-terminal SUMO- and His-tags, expressed in E. coli.

Human USP33 Core Domain recombinant protein (Genbank Accession No. Q8TEY7), a.a. 181-712, with N-terminal SUMO- and His-tags, expressed in E. coli.

Human USP51 recombinant protein (Genbank Accession No. Q70EK9), with N-terminal SUMO- and His-tags, expressed in E. coli.

Human Yod1 recombinant protein, with His-tag, expressed in E. coli.

Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC). Upon incubation with a protease recognizing Ubiquitin, such as USP2 or UCHL3, AMC is released and the increase in fluorescence can be measured using Ex380 nm and Em460 nm wavelengths. This protein contains no extraneous tags.

Ubiquitin-rhodamine 110 is a quenched, fluorescent substrate for deubiquitylases, especially ubiquitin C-terminal hydrolases. Cleavage of the amide bond between the C-terminal glycine of ubiquitin and rhodamine results in an increase in rhodamine fluorescence at 535 nm (Exc. 485 nm).

Human Calmodulin 1, also known as CALM1, CALML2, CAMI, DD132, PHKD, GenBank Accession No. NM_006888, full-length with an N-terminal His-tag, expressed in an E. coli cell expression system. MW = 17 kDa.

Human fibronectin 1 (FN1, also known as CD36), Genbank Accession number X02761.1, a.a. 2175 - 2386 with a C-terminal His-tag, expressed in an HEK293 cell expression system. MW =28 kDa. This protein migrates at a higher than expected molecular weight due to glycosylation.