Antibodies

This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.

Mouse polyclonal antibody raised against a full-length human MCM10 protein.

Mouse polyclonal antibody raised against a full-length human FRMD8 protein.

Mouse polyclonal antibody raised against a full-length recombinant TRAPPC5.

Mouse monoclonal antibody raised against native CD14.

Boster Bio Anti-RAB27A Antibody Picoband™ (monoclonal, 2F5) catalog # M01608. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Boster Bio Anti-alpha 1 Catenin/CTNNA1 Antibody Picoband™ (monoclonal, 10I2) catalog # M01617-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag is affinity purified and specific for rabbit immunoglobulins. Minimal cross reactivity with mouse, rat, human, bovine, guinea pig, and donkey IgG is observed. Use of a secondary antibody in CUT&Tag aids in signal amplification by increasing bound IgG per target epitope [1]. For best results, use with CUTANA™ pAG-Tn5 (EpiCypher 15-1017 & 15-1117). For detailed instructions for use, see EpiCypher’s CUTANA™ CUT&Tag protocol: epicypher.com/protocols. *Mixed Monoclonal: a pool of multiple recombinant monoclonal antibodies.

This antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols (epicypher.com/protocols) and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. SNF2H/SMARCA5 antibody produces CUT&RUN peaks above background (Figure 1) that overlap with H3K4me3 (Figures 1-2), consistent with its known role as the ATP-dependent helicase subunit of the ISWI chromatin remodeler complex [1].