Assays & Kits

The Sensitive EdU Cell Proliferation Kits combine all the advantages of their very popular predecessors – the EdU Cell Proliferation Kits – with a brand new fluorescence enhancer system. Therefore, they are just as easy to handle and as reliable, while showing outstanding efficiency and greatly enhanced signal and sensitivity. Profit from: » Exceptional sensitivity and enhanced fluorescence signals » Lower copper concentrations needed » Better signal-to-noise ratio » Optimized for low content HTS and Flow Cytometry analysis We offer these Sensitive EdU Cell Proliferation Kits for three different ranges of application: cell proliferation analysis by imaging (IM), by flow cytometry (FC) and by high throughput screening analysis (HTS). The IM kits are available for the following wavelenghts: 488 nm and 647 nm The FC kits are available for the following wavelenghts: 488 nm and 647 nm The HTS kits are available for the following wavelenghts: 488 nm and 555 nm

The Sensitive EdU Cell Proliferation Kits combine all the advantages of their very popular predecessors – the EdU Cell Proliferation Kits – with a brand new fluorescence enhancer system. Therefore, they are just as easy to handle and as reliable, while showing outstanding efficiency and greatly enhanced signal and sensitivity. Profit from: » Exceptional sensitivity and enhanced fluorescence signals » Lower copper concentrations needed » Better signal-to-noise ratio » Optimized for low content HTS and Flow Cytometry analysis We offer these Sensitive EdU Cell Proliferation Kits for three different ranges of application: cell proliferation analysis by imaging (IM), by flow cytometry (FC) and by high throughput screening analysis (HTS). The IM kits are available for the following wavelenghts: 488 nm and 647 nm The FC kits are available for the following wavelenghts: 488 nm and 647 nm The HTS kits are available for the following wavelenghts: 488 nm and 555 nm

baseclick’s ClickTech Library Kit full-length mRNA_Seq V2.0 has been developed as a method for sequencing total mRNA of cells for transcriptome analysis. The kit provides reagents for cDNA synthesis from any RNA pool and introduces a single azide nucleotide at the 3’End of cDNA. This azido-modified cDNA can react with an 5’-alkyne-modified adapter sequence in a highly specific and biorthogonal manner under benign click reaction conditions. With the included specially designed PCR Primers, the 3’-adapter-clicked cDNA can be amplified via PCR, enabling subsequent NGS experiments or alternatively targeted gene amplifications

This ClickTech Oligo Link Kit can be used for e.g.: a) click-labeling of Oligonucleotides. b) ligating two Oligonucleotides. Compared to the enzymatic ligation “click ligation” exhibits several advantages: no byproducts, increased ligated product and easy purification of the desired product. Furthermore, several enzymes as well as a broad range of Taq polymerases accept the triazole linkage.

This ClickTech Oligo Link Kit can be used for e.g.: a) click-labeling of Oligonucleotides. b) ligating two Oligonucleotides. Compared to the enzymatic ligation “click ligation” exhibits several advantages: no byproducts, increased ligated product and easy purification of the desired product. Furthermore, several enzymes as well as a broad range of Taq polymerases accept the triazole linkage.

baseclick’s ClickTech PCR Modification Kit contains all reagents required for generating highly labelled PCR products via an easy-to-handle two-step method based on click chemistry. The Mixture of unmodified dNTP and alkyne modified- (EdUTP) nucleotides can be used in PCR reactions with the optimized baseclick Ethynyl Polymerase. The resulting PCR products contain alkyne moieties, which react with azido-derivatives of fluorescent dyes, haptnes and other labels in a highly selective fashion under benign click reaction conditions. We offer within this kit click reagents and a clickable dye of your choice (green 488, yellow 555, orange 594 or red 647) obtaining fluorescent labeled PCR products.

baseclick’s ClickTech PCR Modification Kit contains all reagents required for generating highly labelled PCR products via an easy-to-handle two-step method based on click chemistry. The Mixture of unmodified dNTP and alkyne modified- (EdUTP) nucleotides can be used in PCR reactions with the optimized baseclick Ethynyl Polymerase. The resulting PCR products contain alkyne moieties, which react with azido-derivatives of fluorescent dyes, haptnes and other labels in a highly selective fashion under benign click reaction conditions. We offer within this kit click reagents and a clickable dye of your choice (green 488, yellow 555, orange 594 or red 647) obtaining fluorescent labeled PCR products.

baseclick’s ClickTech PCR Modification Kit contains all reagents required for generating highly labelled PCR products via an easy-to-handle two-step method based on click chemistry. The Mixture of unmodified dNTP and alkyne modified- (EdUTP) nucleotides can be used in PCR reactions with the optimized baseclick Ethynyl Polymerase. The resulting PCR products contain alkyne moieties, which react with azido-derivatives of fluorescent dyes, haptnes and other labels in a highly selective fashion under benign click reaction conditions. We offer within this kit click reagents and a clickable dye of your choice (green 488, yellow 555, orange 594 or red 647) obtaining fluorescent labeled PCR products.

baseclick’s ClickTech PCR Modification Kit contains all reagents required for generating highly labelled PCR products via an easy-to-handle two-step method based on click chemistry. The Mixture of unmodified dNTP and alkyne modified- (EdUTP) nucleotides can be used in PCR reactions with the optimized baseclick Ethynyl Polymerase. The resulting PCR products contain alkyne moieties, which react with azido-derivatives of fluorescent dyes, haptnes and other labels in a highly selective fashion under benign click reaction conditions. We offer within this kit click reagents and a clickable dye of your choice (green 488, yellow 555, orange 594 or red 647) obtaining fluorescent labeled PCR products.