Assays & Kits

The Chemi-Verse™ IKKβ Kinase Assay Kit is designed to measure IKKβ (inhibitor of nuclear factor kappa B kinase subunit beta) serine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified IKKβ, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ TNIK Kinase Assay Kit is designed to measure TNIK (TRAF2 and NCK interacting kinase) serine-threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified TNIK, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ ULK1 Kinase Assay Kit is designed to measure ULK1 (Unc-51-like autophagy-activating kinase 1) serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified ULK1, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ Wee1 Kinase Assay Kit is designed to measure Wee1 serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified Wee1 (amino acids 215-646(end)), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Human IgG Chemiluminescent ELISA Kit is designed to detect and quantify protein levels of human captured by pre-matched antibody pairs. This assay kit comes in a convenient 96-well format, with a pre-coated IgG-specific antibody plate, enough recombinant purified IgG1 standard, and detection reagents for 100 wells.

The IgG Colorimetric ELISA Kit is designed to detect and quantify protein levels of human IgG captured by pre-matched antibody pairs. This assay kit comes in a convenient 96-well format, with a pre-coated IgG-specific antibody plate, enough recombinant purified IgG1 standard, and detection reagents for 100 wells.

The BSA Removal Kit is used for purifying antibodies that do not require buffer replacement and can be directly used for subsequent labeling of reduced antibodies.

Triose-phosphateisomerase (TPI) in plant chloroplasts is an important enzyme involved in calvin cycle in photosynthesis. Acting on the transformation between glyceraldehyde phosphate and dihydroxyacetone phosphate, dihydroxyacetone phosphate can quickly penetrate the chloroplast envelope and enter the cytoplasm, where it is gradually transformed into sucrose. CheKine™ Micro Triose-Phosphate Isomerase (TPI) Activity Assay Kit can detect plant tissues In this kit, TPI converts dihydroxyacetone phosphate into glyceraldehyde-3-phosphate, and glyceraldehyde-3-phosphate and NAD react with glyceraldehyde-3-phosphate dehydrogenase to generate glyceric acid-3-phosphate and NADH. The absorbance change at 340 nm reflects the activity of TPI.

β-Xylosidase (EC 3.2.1.37) exists in organisms such as plants, bacteria, and fungi. It is a key enzyme that catalyzes the degradation of xylan-based hemicelluloses, and the product xylose can serve as a carbon source for microbial fermentation. Additionally, β-xylosidase can be used as a biobleaching agent in the paper industry, offering environmental benefits over traditional bleaching methods and possessing broad application value. CheKine™ Mirco β-Xylosidase (β-X) Activity Assay Kit offers a simple, convenient, and rapid approach for assessing β-Xylosidase activity, which is suitable for plant tissue, bacteria, and fungi samples. The principle involves β-xylosidase catalyzing the production of p-nitrophenol from p-nitrophenyl-β-D-xyloside. p-Nitrophenol exhibits a characteristic absorption peak at 405 nm. By measuring the rate of increase in absorbance at 405 nm, the β-xylosidase activity can be calculated.