Assays & Kits

U0261G is a fusion of the complete AKAP18a protein and the cADDis cAMP biosensor. In HEK 293 cells, the AKAP18a-targeted cADDis sensor appears to be localized to the plasma membrane and intracellular membranes. This assay is genetically encoded and packaged in Big Sky BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl).

U0262G is a fusion of the complete AKAP18b protein to the cADDis biosensor for cAMP. In HEK293T cells the fluorescence is targeted to the plasma membrane. This assay is genetically encoded and packaged in Big Sky BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl).

U0263G is a fusion of the complete AKAP1 protein to the cADDis cAMP biosensor. In HEK293 cells, the AKAP1-targeted cAMP sensor localizes to the mitochondria. This assay is genetically encoded and packaged in Big Sky BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl).

U0264G is a fusion of the complete AKAP79 protein to the cADDis biosensor. This assay is genetically encoded and packaged in Big Sky BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl).

U0266G is a fusion of the complete AKAP12 protein and the cADDis biosensor. In HEK293 cells the fluorescence is targeted to the plasma membrane. This assay is genetically encoded and packaged in Big Sky BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl).

U0267G is a fusion of the complete smAKAP protein and the cADDis biosensor. In HEK293 cells the fluorescence is targeted to the plasma membrane. This assay is genetically encoded and packaged in Big Sky BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl).

Measure diacylglycerol (DAG) signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the DAG sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Increasing green fluorescence indicates DAG increase. These assays are genetically encoded and packaged in Montana Molecular's improved Big Sky BacMam vector for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the DAG sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O).

Measure Calcium signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the green fluorescent G-GECO Calcium sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in an improved Big Sky BacMam vector for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the G-GECO sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O).

Measure Calcium signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the red fluorescent R-GECO Calcium sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in an improved Big Sky BacMam vector for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the R-GECO sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O).