Assays & Kits

A fluorogenic, acetylated peptide substrate for HDAC2, HDAC3, and HDAC6 (tested so far). This substrate fluoresces at excitation 350-380 nm, emission at 440-460 nm. It should be used in conjunction with HDAC Developer, Cat. # 50030.

Please note this product may be subject to fees, we invite you to contact your local office. The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features: <br />• Sensitive - highly sensitive detection of firefly luciferase activity. <br />• Stable - the signal output is stable for more than two hours, providing flexibility with regard to incubation time <br />• Convenient - simple one-step, homogeneous protocol. <br />• High-throughput - one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications <br />• Compatibility

For the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 180 µg RNA from 1 µg DNA in 30 minutes.