Assays & Kits

The PRMT6 Homogeneous Assay Kit is designed to measure the activity of PRMT6 for screening and profiling applications, using AlphaLISA® technology. The key to the PRMT6 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT6 enzyme is incubated with the biotinylated substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alphacounts.

The PRMT8 Homogeneous Assay Kit is designed to measure the activity of PRMT8 for screening and profiling applications, using AlphaLISA® technology. The key to the PRMT8 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT8 enzyme is incubated with the biotinylated substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

The EZH2 Homogeneous Assay Kit is designed to measure activity of the EZH2 for screening and profiling applications, using AlphaLISA® technology. The key to the EZH2 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing EZH2 enzyme is incubated with the histone substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

The SETD2 Direct Activity Chemiluminescent Assay Kit is designed to measure SETD2 activity for screening and profiling applications. The key to the SETD2 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K36 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

The PRMT8 Chemiluminescent Assay Kit is designed to measure PRMT8 activity for screening and profiling applications. The key to the PRMT8 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

The SMYD3 Homogeneous Assay Kit is designed to measure SMYD3 activity for screening and profiling applications. SMYD3 is a histone methyltransferase that promotes diand trimethylation of lysine4 of histone H3. It also exhibits methylation activity toward a lysine residue of MAPK/ERK Kinase Kinase 2 (MAP3K2). The key to the SMYD3 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing SMYD3 enzyme is incubated with the substrate for three hours. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

The EZH2-EED Binding Assay Kit is designed to measure the inhibition of the protein-protein interaction between EZH2 and EED. The key to this kit is the specific binding of EZH2 to EED. With this kit, only two simple steps on a microtiter plate are required. First, incubate a sample containing EZH2, EED and an inhibitor of choice. Next, incubate with a mixture of acceptor and donor beads, followed by reading the Alpha-counts.

The EZH2 (A738T) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (A738T)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes. The EZH2 (A738T) Chemiluminescent Assay Kit comes in a convenient format, with wells precoated with histone H3 peptide substrate, an antibody against methylated K27 residue of Histone H3, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified EZH2 (A738T) complex for 100 enzyme reactions. The key to the EZH2 (A738T) Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrat

The PRMT4 Homogeneous Assay Kit is designed to measure PRMT4 activity for screening and profiling applications. PRMT4 (CARM1) is an arginine methyltransferase and dual functional coregulator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3. The PRMT4 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with His-tagged PRMT4 substrate, primary antibody, methylation assay buffer, and purified PRMT4 for 384 enzyme reactions. The key to the PRMT4 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT4 enzyme is incubated with the substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.