Assays & Kits

The PD-1:PD-L1 TR-FRET Assay is designed to measure the inhibition of PD-1 binding to PD-L1 in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing europium-labeled (Eu) PD-1, dye-labeled acceptor, biotin-labeled PD-L1, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.

he TDO2 Fluorogenic Inhibitor Screening Assay Kit (Human) is designed to measure TDO2 (tryptophan 2,3-dioxygenase 2) activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough recombinant TDO2, fluorogenic substrate and solution, and assay buffer for 100 enzyme reactions. TDO2 converts the conversion of tryptophan to N-formylkynurenine (NFK), which can react with the TDO Fluorogenic reagent present in the TDO Fluorogenic Reaction Solution and generate a green fluorescent molecule. The increase in fluorescence is proportional to TDO2 activity.

The Mouse IDO1 Inhibitor Screening Assay Kit is designed to measure inhibition of the murine IDO1 enzyme. This kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at l=320-325 nm.

The IDO2 Inhibitor Screening Assay Kit is designed to measure mouse IDO2 enzyme inhibition. The Mouse IDO2 Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at l=320 - 325 nm. BACKGROUND: L-tryptophan

The CD47:SIRP-α?[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD47:SIRP-α? interaction. The key to this kit is the high sensitivity of detection of biotinlabeled SIRP-α? by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD47 is coated on a 96-well plate. Next, SIRP-?α-biotin is incubated with CD47 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

The OX40[Biotinylated]:OX40L Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of OX40:OX40L interaction. The key to this kit is the high sensitivity of detection of biotinlabeled OX40 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, OX40L is coated on a 96-well plate. Next, OX40-biotin is incubated with OX40L on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

The IDO1 Fluorogenic Inhibitor Screening Assay Kit is designed to measure enzyme inhibition of indoleamine 2,3-dioxygenase 1 (IDO1). The IDO1 Fluorogenic Inhibitor Screening Assay Kit is simple to use and detects fluorescence at long wavelengths, which minimizes potential errors due to compound interference. In the assay, the inhibitor and enzyme are added to a sample containing L-Trp substrate. After a 1 hour incubation at room temperature, the fluorescence solution is added and incubated at 37°C for four hours. Activity is measured by reading sample fluorescence at λ=510 nm following excitation of the reaction product at λ=400 nm.

The TDO2 Fluorogenic Inhibitor Screening Assay Kit – 384 (Human) is designed to measure TDO2 (tryptophan 2,3-dioxygenase 2) activity for screening and profiling applications. The assay kit comes in a convenient 384-well format, with enough recombinant TDO2, fluorogenic substrate and solution, and assay buffer for 400 enzyme reactions. TDO2 converts the conversion of tryptophan to N-formylkynurenine (NFK), which can react with the TDO Fluorogenic reagent present in the TDO Fluorogenic Reaction Solution and generate a green fluorescent molecule. The increase in fluorescence is proportional to TDO2 activity.

The IDO1 Inhibitor Mechanism of Action (MoA) Assay Kit is designed to determine the mechanism of IDO1 enzyme inhibition (e.g., reversible or irreversible inhibition). The IDO1 Mechanism of Action Assay Kit is simple to use. Inhibitor and enzyme are preincubated allowing the inhibitor to bind. After preincubation, the mixture is diluted into reaction buffer containing the L-tryptophan (L-Trp) substrate and all necessary coupled reaction components. This allows reversible inhibitors to dissociate, while irreversible inhibitors remain associated with the IDO1 enzyme. IDO1 activity is determined by measuring the absorption of the reaction product at ?=320 - 325 nm.