Assays & Kits

Fluorogenic Standard for the determination of 3CL protease (Main protease, Mpro) Activity. Corresponds to the Nsp5 cleavage sequence of SARS-CoV and SARS-Cov-2.

The key to the ADAM10 Fluorogenic Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for ADAM10 reactions. A sample containing ADAM10 is incubated in a reaction mixture with the fluorogenic substrate and fluorescence (λex=485±10 nm, λem=530±10 nm) is measured using a plate reader.

Severe acute respiratory syndrome (SARS) is a viral respiratory illness caused by a corona virus called SARS-CoV-1. It was first reported in February 2013 in Asia and the epidemic affected ~ 26 countries and resulted in more than 8000 deaths. Like SARS-CoV-2, SARS-CoV-1 Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-1 and ACE2 may offer some protection against the viral infection. The SARS-CoV-1 Spike Trimer (S1+S2):ACE2 Inhibitor Screening Assay Kit includes the SARS-CoV-1 Spike protein in its native trimeric conformation to provide a more physiologically relevant screen for inhibitors as well as a compatible platform to investigate the specificity of SARS-CoV-2:ACE2 inhibitors. <br />The key to this kit is that the SARS-CoV-1 Spike Trimer (S1+S2) protein provi

The IL-23R:IL-23A[Biotynilated] Inhibitor Screening Assay Kit is designed to measure the binding of IL-23R (interleukin 23 receptor) to IL-23A (IL-23 subunit alpha) for screening and profiling applications. The IL-23R:IL-23A[Biotinylated] Inhibitor Screening Assay Kit comes in a convenient 96-well format, with enough recombinant purified biotinylated IL-23A, IL-23R, blocking and assay buffer and detection reagents for 100 enzyme reactions.

The 3CL Protease Assay Kit is designed to measure 3CL Protease activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps. 3CL inhibitor GC376 is also included as an inhibitor control.

The Fluorogenic MMP12 Assay Kit is designed to measure MMP12 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps. 

The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Receptor Binding Domain (RBD) of Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection. <br />The Spike RBD (SARS-CoV-2):ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors of this interaction. The key to this kit is the high sensitivity of detection of ACE2-Biotin protein by Streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, Spike RBD protein is attached to a 96-well

The FLT1 Kinase Assay Kit is designed to measure FLT1 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent. 

The Fluorogenic MMP11 Assay Kit is designed to measure MMP11 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.