Assays & Kits

The Spike S1 (B.1.1.7 Variant) (SARS-CoV-2): ACE2 Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling inhibitors of the interaction of ACE2 with the B.1.1.7 Variant of the SARS-CoV-2 Spike S1 protein. The key to this kit is the high sensitivity of detection of ACE2-Biotin protein by Streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, Spike S1 B.1.1.7 protein is coated on a 96-well transparent plate. Next, ACE2-Biotin is incubated with Spike S1 variant on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a luminometer or microplate reader capable of reding chemiluminescence.

The Spike S1 (B.1.1.7 Variant) (SARS-CoV-2):ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors of the interaction of ACE2 with the B.1.1.7 variant of the SARS-CoV-2 Spike S1 protein. The key to this kit is the high sensitivity of detection of ACE2-Biotin protein by Streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, Spike S1 B.1.1.7 protein is coated on a 96-well transparent plate. Next, ACE2-Biotin is incubated with Spike S1 variant on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.  

The SARS-CoV-2 Spike Trimer (S1+S2) (B.1.351 Variant):ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors or neutralizing antibodies of this interaction. The key to this kit is that the SARS-CoV-2 Spike Trimer (S1+S2) (B.1.351 Variant) protein provides a more biologically relevant model than monomeric Spike RBD protein for the investigation of SARS-CoV-2/host cell interaction. Only a few simple steps on a microtiter plate are required for the assay. First, SARS-CoV-2 Spike Trimer (S1+S2) (B.1.351 Variant) is coated on a 96-well plate overnight. Next, the proteins are blocked and pre-incubated with the inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-ACE2, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which then can be quenched and measured using a UV/Vis microplate reader.

The SARS-CoV-2 Spike Trimer (S1+S2) (B.1.1.7 Variant):ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors or neutralizing antibodies of this interaction. The key to this kit is that the SARS-CoV-2 Spike Trimer (S1+S2) (B.1.1.7 Variant) protein provides a more biologically relevant model than monomeric Spike RBD protein for the investigation of SARS-CoV-2/host cell interaction. Only a few simple steps on a microtiter plate are required for the assay. First, SARS-CoV-2 Spike Trimer (S1+S2) (B.1.1.7 Variant) is coated on a 96-well plate overnight. Next, the proteins are blocked and pre-incubated with the inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-ACE2, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which then can be quenched and measured using a UV/Vis microplate reader.

The Pig CD38 Inhibitor Screening Assay Kit (Hydrolase Activity) is designed to measure the glycohydrolase activity of porcine CD38 for screening and profiling applications. In addition, the kit includes the CD38 inhibitor apigenin for use as a control inhibitor.

The ROS1 Kinase Assay Kit is designed to measure ROS1 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The signal is measured using a luminometer or a microplate reader capable of reading chemiluminescence.

The JAK2 JH2 Pseudokinase Domain Inhibitor Screening Assay Kit is designed for screening and profiling small molecules that displace the fluorescently labeled probe (JH2 probe 1) from the JH2 domain of JAK2. The assay is a fluorescence polarization (FP) assay based on the competition of the test compound with the JH2 probe for binding to purified JAK2 JH2.<br /><br />JH2 probe 1 is a small molecule probe that binds to the ATP-binding site of JAK2 pseudokinase domain JH2. The probe has been labeled with a fluorescent tracer, so that binding of the probe to much larger JH2 results in noticeable increases in fluorescence polarization compared to the unbound fluorescent probe (depolarized). Competition between a test drug and the probe results in changes in polarization. Using this kit, only one simple step on a microplate is required for screening. The JH2 probe 1 is incubated with a sample containing JAK2 JH2 to produce a change in fluorescent polarization that can then be measured usin

The JAK2 JH2 Pseudokinase Domain Inhibitor Screening Assay Kit is designed for screening and profiling small molecules that displace the fluorescently labeled probe (JH2 probe 1) from the JH2 domain of JAK2.  The assay is a fluorescence polarization (FP) assay based on the competition of the test compound with the JH2 probe for binding to purified JAK2 JH2.  JH2 probe 1 is a small molecule probe that binds to the ATP-binding site of JAK2 pseudokinase domain JH2. The probe has been labeled with a fluorescent tracer, so that binding of the probe to much larger JH2 results in noticeable increases in fluorescence polarization compared to the unbound fluorescent probe (depolarized). Competition between a test drug and the probe results in changes in polarization. Using this kit, only one simple step on a microplate is required for screening. The JH2 probe 1 is incubated with a sample containing JAK2 JH2 to produce a change in fluorescent polarization that can then be measured using a fluo

The Spike Trimer (S1+S2) (P.1; Gamma Variant) (SARS-CoV-2): ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors or neutralizing antibodies of  the interaction between SARS-CoV-2 Spike and human ACE2. The key to this kit is that the Spike Trimer (S1+S2) (P.1 Variant) (SARS-CoV-2) protein provides a biologically relevant model for the investigation of SARS-CoV-2/host cell interaction. The assay requires only a few steps. First, Spike Trimer (S1+S2) (Gamma Variant) (SARS-CoV-2) is coated on a 96-well plate overnight. After blocking, the protein is pre-incubated with the inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-ACE2, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can be quenched and measured using a UV/Vis microplate reader.