Assays & Kits

The EZH2 (A738T) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (A738T)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes.

The EZH2 (Y641F) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641F)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes.

The EZH2 (Y641N) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641N)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes.

The CD39 Inhibitor Screening Assay Kit is designed to measure CD39 activity for screening and profiling applications. The CD39 assay kit comes in a convenient 96-well format, with purified recombinant CD39 enzyme, ATP, CD39 assay buffer, and Colorimetric detection reagent for 96 enzyme reactions. In addition, the kit includes the CD39 inhibitor POM-1 for use as an inhibitor control.

Please note this product may be subject to fees, we invite you to contact your local office. The Myc Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Myc signaling pathway in cultured HCT116 cells. The Myc Transient Pack contains transfection-ready vectors containing firefly luciferase as a Myc pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HCT116 cells. .The key to the Myc Transient Pack is the expression vectors for c-Myc and Myc luciferase reporter vector. Inside the cells, c-Myc will bind to Max, translocate to the nucleus, and induce expression of the Myc luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized Myc responsive elements located upstream of a

Please note this product may be subject to fees, we invite you to contact your local office. The Notch1/CSL Transient Packis designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Notch signaling pathway in HEK293 cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a Notch pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells. The key to the Notch1/CSL Transient Pack is the expression vector for NOTCH1 that has a deletion of the entire extracellular domain (Notch1DE). Inside the cells, the NOTCH1 DE can be cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. Th

Please note this product may be subject to fees, we invite you to contact your local office. The PDE4D Transient Pack is designed to provide the tools necessary for transiently transfecting and for screening inhibitors of PDE4D7 in cultured HEK293 cells. The assay is based on transfecting cells with the CRE luciferase reporter. CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. Forskolin is commonly used to raise the intracellular level of cAMP in cell physiology studies. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter. However, when cells are co-transfected with PDE4D7 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression

The CD38 Inhibitor Screening Assay Kit (Hydrolase Activity) is designed to measure the glycohydrolase activity of CD38 for screening and profiling applications. The CD38 assay kit comes in a convenient 96-well format, with purified recombinant CD38 enzyme, its substrate N6-etheno-NAD (ε-NAD), and CD38 assay buffer for 100 enzyme reactions. In addition, the kit includes the CD38 inhibitor apigenin for use as a control inhibitor.

The Homogeneous SHP-2 Assay Kit is a complete assay system designed to measure SHP-2 activity for screening and profiling applications. Using this kit, only one simple step on a microtiter plate is needed to analyze the SHP-2 activity level. The fluorogenic substrate, DiFMUP, is incubated with purified SHP-2 and the enzymatic activity releases DiFMU fluorophore that can then be measured using a fluorescence reader.