Assays & Kits

c-Raf (Raf-1; proto-oncogen c-Raf) is a member of the Raf (rapidly accelerated fibrosarcoma) kinase family that plays an important role in regulating the MEK and ERK pathways. The c-Raf Kinase Assay Kit is designed for measuring c-Raf activity for screening and profiling applications. The c-Raf Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant c-Raf enzyme, c-Raf substrate, ATP and kinase assay buffer for 100 enzyme reactions.

Human Casitas B-lineage lymphoma proto-oncogene b (CBL-B) is an E3 ubiquitinprotein ligase that functions as a negative regulator of T-cell activation. It is a promising drug target in cancer immunotherapy. The CBL-B TR-FRET Assay Kit is designed to measure CBL-B auto-ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled Ubiquitin and a terbium-labeled anti-CBL-B antibody to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.

The Abelson (ABL) family of nonreceptor tyrosine kinases is critical to the progression and treatment of chronic myeloid leukemia (CML). The single-point mutation T315I has been linked to drug resistance and poor prognosis, making it an important drug target. The ABL (T315I) Assay Kit is designed to measure ABL activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The ABL (T315I) Assay Kit comes in a convenient 96-well format, with enough purified recombinant ABL enzyme, ABLtide peptide, ATP, and kinase assay buffer for 100 enzyme reactions.

The BCL2L2 TR-FRET Assay Kit is designed to measure the inhibition of BCL2L2 (BCL-w) binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BCL2L2, peptide ligand, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.

TYRO3 is a member of the TAM receptor kinases and plays an important role in cell proliferation/survival, adhesion/migration, and regulation of inflammatory cytokine release. Along with other members of this family, Axl and c-Mer (MERTK), TYRO3 is part of an emerging class of innate immune checkpoints participating in anti-tumoral immunity. Thus, it has been suggested that TYRO3 inhibition could be a promising immunotherapeutic approach in cancer treatment. The TYRO3 Assay Kit is designed to measure TYRO3 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

The GLS1 Inhibitor Screening Assay Kit is designed to measure the hydrolase activity of GLS1 for screening and profiling applications.

Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. CDKs are active only when bound to their regulator proteins, cyclins. CDK activity is tightly controlled for successful cell division. Since abnormal cell division represents cancer pathology, controlling CDK activity has been shown as a promising therapeutic strategy. In particular, CDK1 plays an important role in mitotic progression. The CDK1 Assay Kit is designed to measure CDK1/CyclinB1 activity for screening and profiling applications, using Kinase-Glo® MAX as a detection reagent.

Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. CDKs are active only when bound to their regulator proteins, cyclins. CDK activity is tightly controlled for successful cell division. Since abnormal cell division represents cancer pathology, controlling CDK activity has been shown as a promising therapeutic strategy. In particular, CDK2 plays an important role in DNA replication. The CDK2 Assay Kit is designed to measure CDK2/CyclinA2 activity for screening and profiling applications, using Kinase-Glo® MAX as a detection reagent.

CDK5 is an unconventional member of the CDK family. It is expressed in post-mitotic neurons, and its activity is regulated by neuron-specific activators p35/p25 and p39 rather than the cyclins. It has been suggested that CDK5 plays several roles in the nervous system, including Tau aggregation and tangle formation. The CDK5 Assay Kit is designed to measure CDK5 activity for screening and profiling applications, using Kinase-Glo® MAX as a detection reagent.