Assays & Kits

The PI3Kδ (p110δ/p85α) Assay Kit is designed to measure PI3Kδ (PI3K-delta) activity for screening and profiling applications, using ADP-Glo® Kinase Assay as a detection reagent.

The PI3Kβ (p110β/p85α) Assay Kit is designed to measure PI3Kβ activity for screening and profiling applications, using ADP-Glo® Kinase Assay as a detection reagent.

The PI3Kγ (p110γ/PIK3R5) Assay Kit is designed to measure PI3Kγ activity for screening and profiling applications, using ADP-Glo® Kinase Assay as a detection reagent.

The FGFR2 Assay Kit is designed to measure FGFR2 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

The LTβR:LIGHT[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of LIGHT binding to LTβR . This kit comes in a convenient 96-well format, with biotin-labeled LIGHT, purified LTβR , streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled LIGHT by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LTβR is coated on a 96-well plate. Next, biotinylated LIGHT is incubated with LTβR on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

The Fluorogenic MALT1 Assay Kit is designed to measure MALT1 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.

The cKIT Assay Kit is designed to measure cKIT activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.

The PD-L1:B7-1 [Biotinylated] Inhibitor Screening Assay Kit (Mouse) is designed for screening and profiling inhibitors of murine PD-L1 binding to murine B7-1. The key to this kit is the high sensitivity of detection of biotin-labeled B7-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L1 is coated on a 96-well plate. Next, biotinylated B7-1 is incubated with PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Platelet-derived growth factor receptor A or PDGFRα has been implicated in regulation of cell growth and survival, apoptosis, and differentiation. It has been identified as a potential target in eosinophilic leukemia cancer, inflammatory breast cancer, and gastrointestinal stromal tumors. The D842V is one of the most predominant of the D842 mutations, which are related to gastrointestinal stromal tumors and are associated with resistance to tyrosine kinase inhibitors. The PDGFRα (D842V) Assay Kit is designed to measure PDGFRα (D842V) activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.