Assays & Kits

The Fluorogenic SHP1 Assay Kit is designed to measure SHP1 activity for screening and profiling applications, in a homogeneous assay with no timeconsuming washing steps. This kit is developed to identify inhibitors of the regulatory region of SHP1.

The JMJD2D Homogeneous Assay Kit is designed to measure JMJD2D activity for screening and profiling applications. JMJD2D, also known as KDM4D, is a JumonjiC (JmjC) domain containing histone lysine demethylase that exhibits demethylation activity toward H3-K₉Me³ and H3-K₃₆Me³. The key to the JMJD2D Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2D enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. Our histone demethylase assays utilize highly specific antibodies that recognize demethylated products. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, fol

The JMJD1B Homogeneous Assay Kit is designed to measure JMJD1B activity for screening and profiling applications. JMJD1B, also known as JHDM2B and KDM3B, is a JumonjiC (JmjC) domain containing histone lysine demethylase that exhibits demethylation activity toward H3-K₉Me³ and H3-K₃₆Me³. TThe key to the JMJD1B Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD1B enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. Our histone demethylase assays utilize highly specific antibodies that recognize demethylated products. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor

Please note this product may be subject to fees, we invite you to contact your local office. The KRAS(G12C) Nucleotide Exchange Assay Kit is designed for screening and profiling of KRAS(G12C) antagonists/inhibitors by using BODIPY-GDP to monitor the GDP or GTP binding status. The kit can be used with two different protocols for greater flexibility, either titrating the inhibitor with fixed GTP concentration or titrating the GTP with fixed inhibitor concentration.

Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and this pathway is exploited by both tumors and viruses. The Monkey PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling pathway in the animal model cynomolgus monkey (Macaca fascicularis).

Ready to use Kinase substrate (50X) STAT5 peptide is a peptide derived from the sequence motif of the STAT5.

Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and this pathway is exploited by both tumors and viruses. The Monkey PD-1[Biotinylated]:PD-L1 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling pathway in the animal model cynomolgus monkey (Macaca fascicularis). The key to this kit is the high sensitivity of detection of biotin-labeled PD-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L1 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

The TBK1 Assay Kit is designed to measure TBK1 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

The Cereblon Ubiquitination Homogeneous Assay Kit is designed to measure Cereblon auto-ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled ubiquitin and proper A-screen beads to complete the pairing. This homogeneous assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.