Assays & Kits

UBCH5c (UBE2D3) is an E2 conjugating ubiquitin protein that functions in the ubiquitination of the tumor-suppressor protein p53. It is a promising drug target in cancer immunotherapy. The UBCH5c TR-FRET Assay Kit is designed to measure ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled Ubiquitin and a terbium-labeled antibody recognizing the His-tagged UBCH5c protein to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.

ATP citrate lyase (ACLY) is an important enzyme in fatty acid synthesis and cancer metabolism. The ACLY Assay Kit is designed to measure ACLY activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent. 

The MMP3 (K45E) Inhibitor Screening Assay Kit is designed to measure the endopeptidase activity of MMP3 (K45E) for screening and profiling applications. 

The Wee1 Assay Kit is designed to measure Wee1 activity for screening and profiling applications using Kinase-Glo® Kinase Assay as a detection reagent. 

The Fluorogenic MMP9 (Q279R) Assay Kit is designed to measure MMP9 (Q279R) activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps. 

The Fluorogenic MMP2 Assay Kit is designed to measure MMP2 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.

The ACE2 Inhibitor Screening Assay Kit is designed to measure the exopeptidase activity of ACE2 for screening and profiling applications. 

The PROTAC Optimization Kit for CDK Kinase-Cereblon Binding is designed for testing and profiling of PROTACs directed against the CDK Kinase family and Cereblon. Cereblon (CRBN) is a Substrate recognition component of the DCX (DDB1-CUL44-Rbx1) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins.<br /><br />The CDK inhibitor Palbociclib is included as a control inhibitor of PROTAC binding to CDK. With this kit, only three simple steps on a microtiter plate are required for PROTAC activity detection. First, a sample containing PROTAC is incubated with CRBN and CDK4 or CDK6 Kinase. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

The GLS2 Inhibitor Screening Assay Kit is designed to measure the hydrolase activity of GLS2 for screening and profiling applications.