Cell-based Assays

Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - 5-HT2A receptor assay. Add the unmodified 5-HT2A receptor and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, 5HT2AR BacMam, GRK3 BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). 10mL and 30mL sizes are available as well, as are Borealis kits for several other receptors. Other Montana Molecular tools including the DAG and Calcium sensors can be used alongside this assay.

Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - Kappa Opioid receptor (KOR) assay. Add the unmodified KOR and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, KOR BacMam, GRK5 BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). 10mL and 60mL sizes are available as well, as are Borealis kits for several other receptors. Other Montana Molecular tools including the cADDis cAMP sensor can be used alongside this assay.

Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - Kappa Opioid receptor (KOR) assay. Add the unmodified KOR and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, KOR BacMam, GRK5 BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). 10mL and 30mL sizes are available as well, as are Borealis kits for several other receptors. Other Montana Molecular tools including the cADDis cAMP sensor can be used alongside this assay.

Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - PAR2 assay. Add the unmodified PAR2 receptor and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, PAR2 BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). 10mL and 60mL sizes are available as well, as are Borealis kits for several other receptors. Other Montana Molecular tools including the DAG and Calcium sensors can be used alongside this assay.

Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - PAR2 assay. Add the unmodified PAR2 receptor and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, PAR2 BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). 10mL and 30mL sizes are available as well, as are Borealis kits for several other receptors. Other Montana Molecular tools including the DAG and Calcium sensors can be used alongside this assay.

Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - Delta Opioid receptor (DOR) assay. Add the unmodified DOR and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, DOR BacMam, GRK5 BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). 10mL and 60mL sizes are available as well, as are Borealis kits for several other receptors. Other Montana Molecular tools including the cADDis cAMP sensor can be used alongside this assay.

Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - Delta Opioid receptor (DOR) assay. Add the unmodified DOR and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, DOR BacMam, GRK5 BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). 10mL and 30mL sizes are available as well, as are Borealis kits for several other receptors. Other Montana Molecular tools including the cADDis cAMP sensor can be used alongside this assay.

Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - LPA1 receptor assay. Add the unmodified LPA1 receptor and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, LPA1R BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). 10mL and 60mL sizes are available as well, as are Borealis kits for several other receptors. Other Montana Molecular tools including the cADDis cAMP and DAG sensors can be used alongside this assay.

Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - LPA1 receptor assay. Add the unmodified LPA1 receptor and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, LPA1R BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). 10mL and 30mL sizes are available as well, as are Borealis kits for several other receptors. Other Montana Molecular tools including the cADDis cAMP and DAG sensors can be used alongside this assay.