Results for Cell-based Assays ( 1227 )
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5 (6) - CFDA, SE are most commonly used for the detection of lymphocyte proliferation, as well as for the detection of proliferation of fibroblasts, NK cells, hematopoietic progenitor cells, and other cells. 5 (6) - CFDA, SE labeled cells exhibit green fluorescence. In addition to flow cytometry for cell proliferation detection, florescence microplate reader can also be used to quantify the number of living cells, or fluorescence microscopy can be used for uniform staining of cell tracing observation.
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Fluo-4 AM penetrates the cell membrane and enters the cell, where it is cleaved by esterases within the cell to form Fluo-4, which is then trapped within the cell. When Fluo-4 exists as a free ligand, it is almost non fluorescent, but when combined with intracellular calcium, it can produce strong fluorescence. Laser confocal microscopy can be used to detect changes in intracellular calcium concentration.