Functional Assays

The Kinetic HDAC1 Assay Kit is a complete assay system designed to measure histone deacetylase 1 (HDAC1) activity kinetically for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC1 activity measurements. In addition, the kit includes purified HDAC1 enzyme and a potent HDAC inhibitor, SAHA, for use as a positive and negative control. The Kinetic HDAC1 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only one simple step for setting up the HDAC1 reaction on a microtiter plate is needed and then the HDAC1 activity level can be measured kinetically using a fluorescence reader.

The Kinetic HDAC2 Assay Kit is a complete assay system designed to measure histone deacetylase 2 (HDAC2) activity kinetically for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC2 activity measurements. In addition, the kit includes purified HDAC2 enzyme and a potent HDAC inhibitor, SAHA, for use as a positive and negative control. The Kinetic HDAC2 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only one simple step for setting up the HDAC2 reaction on a microtiter plate is needed and then the HDAC2 activity level can be measured kinetically using a fluorescence reader.

The Kinetic HDAC3 Assay Kit is a complete assay system designed to measure histone deacetylase 3 (HDAC3) activity kinetically for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC3 activity measurements. In addition, the kit includes purified HDAC3/NCOR2 enzyme complex and a potent HDAC inhibitor, SAHA, for use as a positive control. The Kinetic HDAC3 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only one simple step for setting up the HDAC3 reaction on a microtiter plate is needed and then the HDAC3 activity level can be measured kinetically using a fluorescence reader.

The EHMT1 (GLP) Direct Activity Chemiluminescent Assay Kit is designed to measure EHMT1, also known as G9a-like protein or GLP activity for screening and profiling applications. The key to the EHMT1 (GLP) Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K9 residue of H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primaryantibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

The MLL1 Complex Direct Activity Chemiluminescent Assay kit is designed to measure MLL1 activity for screening and profiling applications, using purified MLL1 and its complex components: WDR5, RbBP5,Ash2, and DPY30. The key to the MLL1 Complex Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K4 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

The NSD2 Direct Activity Chemiluminescent Assay Kit is designed to measure NSD2 activity for screening and profiling applications. The key to the NSD2 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S- adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

The NSD1 Direct Activity Chemiluminescent Assay Kit is designed to measure NSD1 activity for screening and profiling applications. The key to the NSD1 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S- adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

The SUV4-20H1 Chemiluminescent Assay Kit is designed to measure SUV4-20H1 activity for screening and profiling applications. The key to the SUV4-20H1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes a methylated residue of Histone H4. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

The SMYD4 Chemiluminescent Assay Kit is designed to measure SMYD4 activity for screening and profiling applications. The SMYD4 Chemiluminescent Assay Kit comes in a convenient format, with 96-well plate precoated with a specific substrate, the antibody against methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, Sadenosylmethionine, methyltransferase assay buffer, and purified SMYD4 enzyme for 96 enzyme reactions. The key to the SMYD4 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemilumine