Cells Reagents

Skeletal Muscle Cell Growth Medium is a sterile liquid culture medium designed for cultivating human skeletal muscle cells (HSKMC). Delivered as a basal medium, it is suitable for culturing Provitro HSKMC upon the addition of supplement mix components. The formulation is optimized for initial seeding of 4,000 cells/cm², facilitating growth up to confluence (approximately 90%). The medium does not require feeder layers, matrix substrates, or additional substances for effective cell cultivation.Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. Skeletal muscle cell growth medium is thoroughly tested after each production. All components are tested in a stringent biological assay. Each batch is checked for human endothelial cells proliferating characteristics. The cells c

Endothelial Cell Growth Medium, Advanced, is a sterile liquid culture medium designed for the cultivation of endothelial cells. Delivered in the form of a basal medium, it is well-suited for culturing Provitro human endothelial cells when supplemented with the provided mix components. The formulation is optimized for the initial seeding of 4,000 cells/cm², allowing for growth up to confluence (approximately 90%). The use of feeder layers, matrix substrates, or other additives is not necessary. However, for freshly isolated cells, we recommend incorporating the antibiotic supplement to address potential reductions in proliferative activity. Endothelial cell proliferation medium is thoroughly tested after each production. All components are tested in a stringent biological assay. Each batch is checked for human endothelial cells proliferating characteristics. The cells cultured in endothelial cell growth medium are checked regarding their morphology, the adherence rate, the colony formin

Microvascular Endothelial Cell Growth Medium, Advanced, is a sterile liquid culture medium designed for cultivating human microvascular endothelial cells (HMVEC). Delivered as a basal medium, it is suitable for culturing Provitro HMVEC upon the addition of supplement mix components. The formulation is optimized for the initial seeding of 4,000 cells/cm², allowing growth up to confluence (approximately 90%). The use of feeder layers, matrix substrates, or additional substances is unnecessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. Microvascular endothelial cell proliferation medium is thoroughly tested after each production. All components are tested in a stringent biological assay. Each batch is checked for human endothelial cells proliferating characteristics. The cells cultured in endothelial cell growth medium are checked regarding their morphology, the adherence rate, the colony forming

Keratinocyte Growth Medium is a sterile liquid serum-free culture medium designed for cultivating human keratinocytes (HKER). Delivered as a basal medium, it is suitable for culturing Provitro HKER upon the addition of the supplement mix. The formulation is optimized for the initial seeding of 6,000 cells/cm², supporting growth up to confluence (approximately 75%). Feeder layers, matrix substrates, or additional substances are not necessary for effective cell cultivation. The Keratinocyte Growth Medium undergoes comprehensive testing after each production cycle. All components undergo rigorous testing in a stringent biological assay. Each batch is specifically examined for the proliferating characteristics of human keratinocytes (HKER). The cells cultured in the keratinocyte growth medium are thoroughly assessed for their morphology, adherence rate, colony-forming efficiency, and population doubling time. Storge: Store the supplementedKreatinocyte

Urothelial Cell Growth Medium is a sterile, serum-free liquid culture medium designed for the cultivation of Human Urothelial Epithelial Cells (HUEPC). Delivered in the form of a basal medium, it becomes a complete and suitable environment for culturing HUEPC upon the addition of three supplement components. The formulation is meticulously optimized to support the initial seeding of 6,000 cells per square centimeter (cm²) up to confluence, reaching approximately 75%. The Urothelial Cell Growth Medium undergoes rigorous quality control measures following each production cycle. Every constituent undergoes thorough testing through a stringent biological assay. Each batch is specifically scrutinized for the proliferating characteristics of Human Urothelial Epithelial Cells (HUEPC). The cells cultured in this medium are meticulously evaluated for various parameters including morphology, adherence rate, colony-forming efficiency, and population doubling time. This meticulous quality assuranc

Immediately after delivery, place the passage kit in the dark at -20°C. Prior use thaw the passage kit. After thawing store the passage kit in the dark at 2°C to 8°C for a maximum of 4 weeks. Take care: After thawing shelf time is limited to 4 weeks! After thawing the passage kit can be stored in the dark at 2°C to 8°C for up to 1 month. Do not heat the kit over 37°C or use uncontrollable sources of heat (e.g. microwave appliances). Do not refreeze the kit. This can lead to high salt concentrations by freezing out pure water which will cause irreversible damage. Overtrypsination causes irreversible damage. Because of the tryptic activity of this passage kit, do not exceed the recommended incubation period. Exposing the cells too long will cause irreversible damages to your culture.

Immediately after delivery, place the passage kit in the dark at -20°C. Prior use thaw the passage kit. After thawing store the passage kit in the dark at 2°C to 8°C for a maximum of 4 weeks. Take care: After thawing shelf time is limited to 4 weeks! After thawing the passage kit can be stored in the dark at 2°C to 8°C for up to 1 month. Do not heat the kit over 37°C or use uncontrollable sources of heat (e.g. microwave appliances). Do not refreeze the kit. This can lead to high salt concentrations by freezing out pure water which will cause irreversible damage. Overtrypsination causes irreversible damage. Because of the tryptic activity of this passage kit, do not exceed the recommended incubation period. Exposing the cells too long will cause irreversible damages to your culture.

Immediately after delivery, place the passage kit in the dark at -20°C. Prior use thaw the tube. After usage store them in the dark at 2°C to 8°C for a maximum of 2 weeks. Take care: After thawing shelf time of Dispase II solution is limited to 14 days! After thawing the passage kit can be stored in the dark at 2°C to 8°C for up to2 weeks. Do not heat the kit over 37°C or use uncontrollable sources of heat (e.g. microwave appliances). Do not refreeze the kit. This can lead to high salt concentrations by freezing out pure water which will cause irreversible damage.

In a serum-free cell culture system, it is very important to cryopreserve cells also in a serum-free freeze medium and not in a serum containing medium. Using an ordinary serum containing freeze medium, the serum affects the cell culture system over a long period. This influence can only be removed by dilution during a large number of passages after seeding. Provitro's Cryo-SFM is a new formulation for cryopreservation of animal and human cells containing no serum, but rather DMSO, methyl cellulose and other cryoprotectant ingredients. After cryopreservation and thawing, a very high percentage of viable cells is obtained. Cells which are cryopreserved in Cryo-SFM also show excellent attachment ability as well as growth performance after thawing. Adherent Cells: Remove adherent cells with Trypsin/EDTA (Provitro Cat.-No.: 204 0001). Neutralize Trypsin with Trypsin inhibitor (Provitro Cat.-No. 204 0001), pellet cells by centrifuging at low acceleration (200 x g) for 3-5 minutes and