Labelling

BP Fluor 680 is a bright and photostable near-IR dye that is often used for small animal imaging applications in the 700 nm wavelength and provides excellent clearance profiles in animals. Flow cytometry is another common application for this dye. BP Fluor 680 ideally suited for the 633 nm laser line. This near-IR fluorescent dye is water soluble and pH-insensitive from pH 4 to pH 10. BP Fluor 680 molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. The long wavelength emission allows for detection in complex samples with auto-fluorescent background signals. TFP (tetrafluorophenyl) ester is an amine-reactive activated ester that reacts with primary amines of biomolecules in the same way as the succinimidyl ester (SE or NHS-ester) to form a stable amide bond. The major advantage of TFP esters over the succinimidyl ester is much improved resistance to spontaneous hydrolysis during conju

BP Fluor 680 is a bright and photostable near-IR dye that is often used for small animal imaging applications in the 700 nm wavelength and provides excellent clearance profiles in animals. Flow cytometry is another common application for this dye. BP Fluor 680 ideally suited for the 633 nm laser line. This near-IR fluorescent dye is water soluble and pH-insensitive from pH 4 to pH 10. BP Fluor 680 molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. The long wavelength emission allows for detection in complex samples with auto-fluorescent background signals. Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds.

BP Fluor 680 is a bright and photostable near-IR dye that is often used for small animal imaging applications in the 700 nm wavelength and provides excellent clearance profiles in animals. Flow cytometry is another common application for this dye. BP Fluor 680 ideally suited for the 633 nm laser line. This near-IR fluorescent dye is water soluble and pH-insensitive from pH 4 to pH 10. BP Fluor 680 molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. The long wavelength emission allows for detection in complex samples with auto-fluorescent background signals. BP Fluor 680 is a carbonyl reactive building block used to modify carboxylic groups in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. It also can be employed as a polar tracer, a reactive dye for labeling proteins via a carboxylic acid moiety, and amine donor for en

Difluorocarboxyfluorescein NHS Ester, 6-isomer (also known as Oregon Green™ 488 Carboxylic Acid, Succinimidyl Ester, 6-isomer) is an amine-reactive fluorinated analog of fluorescein that overcomes some of the key limitations of fluorescein, including greater photostability and a lower pKa (pKa ~ 4.7 versus 6.4 for fluorescein), making its fluorescence essentially pH insensitive in the physiological pH range. Although the mixed isomers of Difluorocarboxyfluorescein NHS Ester, is a preferred, routinely used fluorescent dye for labeling proteins, peptides and nucleotides, purification of peptide and nucleotides labeled with 5(6) isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer usually give better resolution in HPLC purification that is often required in the conjugation processes. Difluorocarboxyfluorescein NHS Ester dye is structurally identical to Oregon Green™ 488 Carboxylic Acid, Succinimidyl E

Difluorocarboxyfluorescein NHS Ester, 5-isomer (also known as Oregon Green™ 488 Carboxylic Acid, Succinimidyl Ester, 5-isomer) is an amine-reactive fluorinated analog of fluorescein that overcomes some of the key limitations of fluorescein, including greater photostability and a lower pKa (pKa ~ 4.7 versus 6.4 for fluorescein), making its fluorescence essentially pH insensitive in the physiological pH range. Although the mixed isomers of Difluorocarboxyfluorescein NHS Ester, is a preferred, routinely used fluorescent dye for labeling proteins, peptides and nucleotides, purification of peptide and nucleotides labeled with 5(6) isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer usually give better resolution in HPLC purification that is often required in the conjugation processes. Difluorocarboxyfluorescein NHS Ester, 5-isomer dye structurally is identical to Invitrogen’s Oregon Green™ 488 Carboxyl

Difluorocarboxyfluorescein Cadaverine, 5-isomer (sold under Oregon Green™ 488 Cadaverine, 5-isomer name) is a carbonyl reactive building block used to modify carboxylic groups in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. It also can be employed as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety. Difluorocarboxyfluorescein Cadaverineis a fluorinated analog of fluorescein that overcomes some of the key limitations of fluorescein, including greater photostability and a lower pKa (pKa ~ 4.7 versus 6.4 for fluorescein), making its fluorescence essentially pH insensitive in the physiological pH range. Difluorocarboxyfluorescein Cadaverine, 5-isomer structurally is identical to Invitrogen’s Oregon Green™ 488 Cadaverine, 5-isomer. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

2′, 7′-Difluorocarboxyfluorescein Azide, 6-isomer (also under Oregon Green 488 Azide (Oregon Green™ 6-Carboxamido-(6-Azidohexanyl), Triethylammonium Salt), 6-isomer name) is a bright, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. 2′,7′-Difluorocarboxyfluorescein fluorinated analog of fluorescein that overcomes some of the key limitations of fluorescein, including greater photostability and a lower pKa (pKa ~ 4.7 versus 6.4 for fluorescein), making its fluorescence essentially pH insensitive in the physiological pH range. Difluorocarboxyfluorescein Azide, 6-isomer is structurally identical to Invitrogen’s Oregon Green® 488 Azide, isomer 6. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Carboxyrhodamine 110 is the nonsulfonated analog of the BP Fluor 488 dye. The amine-reactive Carboxyrhodamine 110 LC can be used to create bright and photostable green-fluorescent bioconjugates with excitation/emission maxima ~502/527 nm. For many applications, the dye is preferred over carboxyfluorescein or FITC because of its exceptional photostability and fluorescence insensitivity to pH (4-9). This reagent is supplied as Boc protected derivative to ensure compatibility with Fmoc solid phase peptide synthesis. The hydrophilic spacer arm contains a hydrophilic, 4-unit, polyethylene glycol (PEG) group imparts water solubility of the labeled peptide resulting in less aggregation when stored in aqueous solution. Spacers of different lengths are available upon request. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

5-Carboxyrhodamine 110 NHS Ester is the nonsulfonated analog of the BP Fluor 488 dye. The amine-reactive 5(6)-Carboxyrhodamine 110 NHS Ester can be used to create bright and photostable green-fluorescent bioconjugates with excitation/emission maxima ~502/527 nm. For many applications, the dye is preferred over 5-(6)-carboxyfluorescein NHS ester or FITC because of its exceptional photostability and fluorescence insensitivity to pH (4-9). Although the mixed isomers of Carboxyrhodamine 110 NHS Ester preferred, routinely used fluorescent dye for labeling proteins, peptides and nucleotides, purification of peptide and nucleotides labeled with 5(6) isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer usually give better resolution in HPLC purification that is often required in the conjugation processes. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.