Labelling

7-(Diethylamino)coumarin-3-carbohydrazide is a fluorescent dye for labeling proteins and nucleic acids. The emission wavelength is 415 nm.

Pacific Blue NHS ester is a fluorescent dye for labeling proteins and nucleic acids. The excitation wavelength is 410 nm and the emission maxima is 455 nm.

DY-680-NHS ester is an amine reactive hydrophilic fluorochrome. It can be conjugated to an antibody with higher D/P ratio without causing fluorescence quenching and conjugate precipitation, and it is widely used in applications including western blotting, microscopy, flow cytometry, and cell-based assays. The spectrum has an excitation of 690nm and emission at 709nm.

CalFluor 647 Azide is a fluorogenic azide probe that will not fluoresce until it is activated through click reactions. The probe is excitated by 633 nm and 647 nm wavelengths. CalFluor 647 Azide is also a water-soluble probe.

Cy5 Biotin Azide is a PEG compound with a Cy5 fluorophore coupled to a terminal biotin group. The Cy5 fluorophore is pH independent between pH 4-10. The biotin group is effective at binding proteins such as binding to avidin, streptavidin or neutravidin. The PEG linker improves the water solubility of the compound and mimizes steric hindrance.

Fluorescein Biotin Azide is a PEG compound with both terminal biotin and azide functional groups, as well as a green-fluorescein moiety. The azide group readily reacts with alkyne, DBCO, BCN through Click Chemistry reactions to form a stable triazole linkage. The biotin group is effective at binding proteins such as binding to avidin, streptavidin or neutravidin. The hydrophilic PEG spacer can increase aqueous solubility of the biotin conjugated molecules. It also can also help minimize steric hindrance and provide extra flexibility with the binding to stravidin and avidin molecules.

Cy5 Azide NHS Ester is a fluorescent probe with terminal NHS ester and azide groups. The Cy5 fluorescent molecule is a water-soluble, far-red-fluorescent probe that is excitated at 633 nm and 647 nm. The azide group can react with alkyne, BCN, DBCO via Click Chemistry to yield a stable triazole linkage. The reaction works well under diluted conditions. The NHS ester can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

Coumarin Azide Plus is a fluroescent agent with a terminal azide group. The fluroescent compound has a max absorption at 340 nm and emission at 440 nm. The Coumarin Azide Plus was designed with a copper-chelating system within its structure, enabling the formation of strong, active copper complexes that act as both reactant and catalyst in CuAAC reactions.

Pyrene Azide Plus is a fluroescent agent with a terminal azide group. The Pyrene Azide Plus was designed with a copper-chelating system within its structure, enabling the formation of strong, active copper complexes that act as both reactant and catalyst in CuAAC reactions. The fluroescent compound has a max absorption at 403 nm and emission at 453 nm.