Enzymes

Recombinant human ROCK1 (a.a. 17-535) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST-tag. The gene accession number is NM_005406.

Recombinant human ROCK2 (a.a. 5-554) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST-tag. The gene accession number is NM_004850.

Recombinant human IRAK2 (GenBank Accession No. NM_001570), full length with N-terminal GST tag, MW= 103 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human MAPKAPK2 (MK2) (GenBank Accession No. NM_032960), full length with N-terminal GST-tag, MW= 72 kDa, expressed in a baculovirus expression system.

Human MKK6 (GenBank Accession No. NM_002758.3), a.a. 2-end, with N-terminal GST-tag, MW=63.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human MAP3K14 (mitogen-activated protein kinase kinase kinase 14, NIK, GenBank Accession No. NM_003954), a.a. 318-947(end) with N-terminal GST-tag, MW=96 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human MAPK14 (GenBank Accession No. NM_139012), full length, with N-terminal GST-tag, MW=65 kDa, expressed in an E. coli expression system.

Human MAPK10 (mitogen-activated protein kinase 10), also known as PRKM10, JNK3 (c-Jun N-terminal kinase-3), or SAPKβ (stress activated protein kinase-β), GenBank Accession No. NM_002753, a.a. 2-422 (end), with N-terminal GST-tag, MW=75 kDa, expressed in Sf9 cells via a baculovirus expression system.

STK33 (GenBank Accession No. NM_030906.2), a.a. 2-end, with N-terminal GST-tag, MW = 84 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.