Enzymes

Human GTF2H2D full-length ORF (NP_001035955.1, 1 a.a. - 395 a.a.) recombinant protein with GST tag at N-terminal.

Human LGALS9C full-length ORF (AAH73889.1, 1 a.a. - 356 a.a.) recombinant protein with GST tag at N-terminal.

Peroxidase Labeling Kit - NH2 is for simple and rapid preparation of peroxidase-labeled IgG for enzyme immunoassays (EIA), immunoblotting or immunostaining and peroxidase-labeled antigen for competitive EIA.

Proteolysis Targeting Chimeras (PROTACs) have emerged as novel therapeutics to target the undruggable proteome. PROTACs are heterobifunctional small molecules that both simultaneously bind to a target protein and a ubiquitin (Ub) E3 ligase. This subsequently leads to ubiquitination and degradation of the target protein. The In vitro ubiquitination kit has been developed to establish a high throughput approach that can accurately predict PROTAC efficiency by monitoring the protein’s intrinsic ability to get ubiquitinated. We offer this kit for three E3 ubiquitin ligases: Cereblon, VHL, and HDM2 to monitor PROTAC mediated ubiquitination for target of choice.

Recombinant human JAK1 (janus kinase 1), encompassing amino acids 561-852 that correspond to the JH2 pseudokinase domain). This construct contains an N-terminal His-tag (6xHis). This protein was affinity purified.

Recombinant human JAK1 (janus kinase 1), encompassing amino acids 561-852 that correspond to the JH2 pseudokinase domain). This construct contains an N-terminal His-tag (6xHis). This protein was affinity purified.

Recombinant human EGFR (epidermal growth factor receptor) encompassing tyrosine kinase domain amino acids 668-1210(end) with a deletion of amino acids 746 to 750 and mutations T790M and C797S. This construct contains an N-terminal GST-tag and a C-terminal His-tag (6xHis). This protein was affinity purified.

Recombinant human KEAP1 (kelch like ECH associated protein 1), full length, encompassing amino acids 2-624. This construct contains an N-terminal His-tag (6xHis). This recombinant protein was affinity purified. 

Human Akt1 (GenBank Accession No. NM_001014432), full length (a.a. 1-480) with N-terminal His-tag, MW=60.2 kDa, expressed in a Baculovirus infected Sf9 cell expression system.