Enzymes

This kit is used to detect DNase contamination in sample.

This kit is used to detect RNase contamination in sample.

This kit is used to detect RNase contamination in sample.

Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase with high specificity for the T7 promoter. This enzyme catalyzes the 5’ to 3’ synthesis of RNA from DNA downstream from the promoter.

Inorganic pyrophosphate (PPi) is generated as a reaction byproduct in many biosynthetic reactions which utilize ATP, including in vitro transcription and DNA polymerization. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate (P₂O₇^-4 + H₂O + PPase 2HPO₄^-2). PPase requires divalent metal cation (Mg²⁺) for the enzymatic activity.

RNase III is ribonuclease III (RNase III) recombinantly expressed by E. coli. This specific exonuclease cleaves double-stranded RNA (dsRNA) and generates 12-35bp dsRNA fragments with 5'-PO4 and 3'-OH, 3' overhangs.

RNase III is ribonuclease III (RNase III) recombinantly expressed by E. coli. This specific exonuclease cleaves double-stranded RNA (dsRNA) and generates 12-35bp dsRNA fragments with 5'-PO4 and 3'-OH, 3' overhangs.

T7 RNA Polymerase is a DNA-dependent RNA polymerase from T7 phage, that possesses a strong and specific 5' to 3' RNA polymerase activity. T7 RNA Polymerase has high specificity for T7 promoter sequences and will synthesize large quantities of RNA from a DNA fragment inserted downstream from a promoter.

T7 RNA Polymerase is a DNA-dependent RNA polymerase from T7 phage, that possesses a strong and specific 5' to 3' RNA polymerase activity. T7 RNA Polymerase has high specificity for T7 promoter sequences and will synthesize large quantities of RNA from a DNA fragment inserted downstream from a promoter.