Enzymes

Human Akt2 (GenBank Accession No. NM_001626), full length with N-terminal His-tag, MW=57 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human Aurora kinase B (GenBank Accession No. NM_004217), a.a. 2-344, with N-terminal GST-tag and C-terminal His-tag, MW=66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human AKT Serine/Threonine Kinase 1 (AKT1), also known as Protein Kinase B Alpha (PKBα), and RAC, GenBank Accession No. NM_001014432, a.a. 1-480(full length) with N-terminal His-tag, was expressed in Sf9 insect cells via a baculovirus expression system. MW=60 kDa. The purified AKT1 is activated in vitro using PDK1 enzyme.

Human Aurora kinase A (GenBank Accession No. NM_003600), a.a. 2-403 with N-terminal His-tag, MW=50 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human BRAF (GenBank accession # NM_004333), a.a. 432-725 with N-terminal His-tag, MW=36 kDa, and p50 (GenBank accession # NM_007065), a.a. 2-378(end) with N-terminal FLAG-tag, MW=45 kDa, coexpressed in a Baculovirus infected Sf9 cell expression system. This protein exists in a complex with Cdc37 and p50.

Human GSK3α (alpha) GenBank Accession No. BC027984), full length, with N-terminal GST-tag, MW=81.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human GSK3β GenBank Accession No. NM_002093), full length, with N-terminal GST-tag and C-terminal His-tag, MW=73 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human RAF1, also known as c-RAF (GenBank Accession No. NM_002880), a.a. 306-648, containing the mutations Y340D and Y341D, with N-terminal GST-tag, MW=65 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Human NEK2 (GenBank Accession No. NM_002497) full length with N-terminal His-tag, MW=53 kDa, expressed in a Baculovirus infected Sf9 cell expression system.