Enzymes

Human v-raf murine sarcoma 3611 viral oncogene homolog, transcript variant 1 (A-RAF1), (GenBank Accession No. NM_001654), a.a. 282-606 (end), containing mutations YY301-302DD, with N-terminal GST-tag and C-terminal His-tag, MW=63 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Recombinant human AKT2 (GenBank Accession No. NM_001626), full length with N-terminal His-tag, MW=60.2 kDa, was expressed in Sf9 insect cells via a baculovirus expression system. The purified AKT2 is activated in vitro using PDK1 enzyme.

Recombinant human AKT3 (GenBank Accession No. NM_005465), a.a. 2-end, with N-terminal His-tag, MW= 60 kDa, expressed in a baculovirus expression system.

Human STK3 (serine/threonine kinase 3), also known as MST-2 (Mammalian STE20-like protein kinase 2) or KRS1, GenBank Accession No. NM_006281, a.a. 2-492(end), with N-terminal GST-tag, MW=78.4 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

Human NEK6 (GenBank Accession No. NM_014397), a.a. 1-313 with N-terminal His-tag, MW=40.1 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human PKC iota (PCKι), also known as PKClambda (PKCλ), GenBank Accession No. NM_002740, a.a. 2-587 with N-terminal GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW= 93 kDa.

Human recombinant BRSK2 (GenBank Accession No. NM_003957), full length with N-terminal His-tag, MW=75 kDa, expressed in expressed in a baculovirus expression system.

Human STK4 (serine/threonine kinase 4), also known as MST-1 (Mammalian STE20-like protein kinase 1) or KRS2, GenBank Accession No. NM_006282, a.a. 2-488(end), with N-terminal GST-tag, MW=81.5 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

Recombinant human activin receptor-like kinase 1, or ALK1, (a.a. 144-end), with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system. The gene accession number is NM_000020.