Enzymes

Recombinant human ASK1 (649-946) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST-tag. The gene accession number is NM_005923.

Recombinant human CAMK4, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.

Recombinant mouse CAMK1, full length, with N-terminal GST-tag, expressed in E. coli cells

Recombinant mouse CAMK1β, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.

Recombinant human CAMK1γ, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.

Human Plk1 (GenBank Accession No. NM_005030), full length with C-terminal His-tag, MW=68 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human Plk2 (GenBank Accession No. NM_006622), full length, with N-terminal GST-tag, expressed in a baculovirus infected Sf9 cell expression system. Theoretical MW is 107 kDa.

Human Plk3 (GenBank Accession No. NM_004073), (a.a. 58-339) with N-terminal His-tag, MW=36.7 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Source: Baculovirus infected Sf9 cells Purity:≥80% by SDS-PAGE. Specific Activity: 500 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of casein substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP.

Recombinant human CAMK1γ (a.a. 1-330), with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.