Enzymes

Human PAK4 (GenBank Accession No. BC002921), a.a. 300-591, MW=33.3 kDa, expressed in E. coli. PAK4 is fully phosphorylated at S474.

Human PAK5 (GenBank Accession No. NM_177990), a.a. 425-719, MW=33.9 kDa, expressed in E. coli. PAK5 is fully phosphorylated at S602 and an additional site

Human MNK (T385D) (GenBank Accession No. NM_003684), a.a. 2-end with deletion a.a. 165-205 and threonine 385 to aspartic acid mutation, and N-terminal GST-tag, MW=74 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human MUSK (GenBank Accession No. NM_005592), a.a. 530-end with N-terminal His-tag, MW = 38.5 kDa, expressed in Baculovirus infected Sf9 cell expression system.

Human 3-phosphoinositide-dependent protein kinase (PDK1), GenBank Accession No. NM_002613, full length with N-terminal GST-tag, MW=89 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human PAK6 (GenBank Accession No. NM_020168), a.a. 385-680, MW=34.2 kDa, expressed in E. coli.

cGMP-dependent Human PKG1α (GenBank Accession No. NM_001098512), full length with N-terminal His-tag, MW = 80 kDa, expressed in Baculovirus infected Sf9 cell expression system.

Human PKG1β (beta) (GenBank Accession No. NM_006258), full length with N-terminal His-tag, MW = 78 kDa, expressed in Baculovirus infected Sf9 cell expression system.

PDHK1, also known as Pyruvate Dehydrogenase Kinase or PDK-1 (GenBank Accession No. NM_002610), a.a. 2-437(end), with an N-terminal GST-tag, MW=71.8 kDa, expressed in Sf9 insect cells via a baculovirus expression system.