Purified by the method of Williams, Sung and Laskowski, J. Biol. Chem., 236, 1130 (1961). Further treated to inactivate contaminating 5'-nucleotidase activity according to Sulkowski and Laskowski: Biochim. Biophys. Acta, 240, 443 (1961). Lyophilized in vials.
Prepared from the 1 mM sodium pyrophosphate, pH 6.9, alumina gel eluate of Hilmoe, Biochem. Prep, 8, (Meister, A., ed.), John Wiley & Sons, NY, NY, p. 105 (1961). Lyophilized in vials.
Prepared from the 1 mM sodium pyrophosphate, pH 6.9, alumina gel eluate of Hilmoe, Biochem. Prep, 8, (Meister, A., ed.), John Wiley & Sons, NY, NY, p. 105 (1961). Lyophilized in vials.
