Lentivirus

GCaMP is a genetically encoded calcium indicator (GECI) and created from a fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin light chain kinase. When GECI binds Ca2+, the conformational change of GCaMP induces proximity of the GFP parts, eliciting strong GFP fluorescence signal, which allows measuring action potentials and other receptor activation events that trigger Ca2+ influx. The advantage of GECI's are that they can be genetically specified for studies in living organisms. GCaMP6 is a novel ultra-sensitive format of GCaMP that outperformed other sensors in terms of accuracy and sensitivity in measuring cytosol Ca2+ level. Mutations in calmodulin part of GCaMP6 created several variants which showed different calcium fluorescence decay rate (fast - GCamP6f, slow - GCaMP6f and medium - GCaMP6m). LV-CaMKII-GCaMP6f is pre-made lentivirus which expresses GCaMP6f under CaMKII promoter for neuronal exclusive expression. Ready to us

Please note this product may be subject to fees, we invite you to contact your local office. Expression Negative Control Lentivirus (Inducible Tet On™) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viral particles do not express any specific protein under a tight TRE tetracycline-inducible promoter but include a G418 selection marker.

Please note this product may be subject to fees, we invite you to contact your local office. Membrane eGFP Lentivirus are replication incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain eGFP (enhanced green fluorescent protein), with a plasma membrane-targeting sequence at the N-terminus of eGFP, under the control of an EF1a promoter. The lentiviruses also transduce a puromycin selection marker. eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry.

Please note this product may be subject to fees, we invite you to contact your local office. Mitochondrial eGFP Lentivirus are replication incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain eGFP (enhanced green fluorescent protein), with a mitochondrial targeting sequence at the N-terminus of eGFP), under the control of an EF1a promoter. The lentiviruses also transduce a puromycin selection marker. eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry.

Please note this product may be subject to fees, we invite you to contact your local office. Endoplasmic Reticulum (ER) eGFP Lentivirus are replication incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain eGFP (enhanced green fluorescent protein), with an ER-targeting and retrieval sequences at both the N and C-terminus of eGFP, respectively, under the control of an EF1a promoter. The lentiviruses also transduce a puromycin selection marker (Figure 1). eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry.

Please note this product may be subject to fees, we invite you to contact your local office. EGFP Beta-Actin Lentivirus are replication incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain eGFP and β-actin (NM_001101.5) (eGFP-Linker-β-actin) driven by an EF1A promoter. The lentiviruses also transduce a puromycin selection marker (Figure 1). eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry.

Please note this product may be subject to fees, we invite you to contact your local office. eGFP-Tubulin Lentivirus are replication incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain eGFP and α-tubulin (eGFP-linker-alpha-tubulin) driven by an EF1A promoter. The lentiviruses also transduce a puromycin selection marker. eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry.

Please note this product may be subject to fees, we invite you to contact your local office. Nuclear mCherry Lentivirus are replication incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain a nuclear mCherry, with nuclear localization sequences (NLS) at both the N- and C-terminus of mCherry, under the control of an EF1a promoter. The lentiviruses also transduce a puromycin selection marker. mCherry expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry.

Please note this product may be subject to fees, we invite you to contact your local office. The Firefly Luciferase-Nuclear eGFP Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain firefly luciferase and nuclear eGFP (Luc2-P2A-nuclear eGFP) driven by an EF1A promoter. These lentiviruses also transduce a puromycin selection marker (Figure 1). The nuclear eGFP has NLS sequences at the C-terminus. eGFP has an excitation wavelength of 488 nm, an emission wavelength of 509 nm, and extinction coefficient of 55,000 M-1cm-1.