Assays & Kits

DNA extraction supporting reagent for tough tissues, such as bone and tooth.

Zinc Assay kit is intended for the quantitative determination of zinc in biological sample by 96-well reader use. Target: zinc (Zn2+). Measuring range: 4 -1,000 μg/Dl. Species: all. Sample: serum, plasma, urine, saliva, tissue extract, cell lysate, biological fluid, food, beverage etc. Apparatus: UV/Vis 96-well reader or spectrophotometer. Wavelength: 560 nm (550-580nm). Procedure: 10 min

This reagent kit is intended for the quantitative determination of zinc in biological sample by 96-well reader use. Target: zinc (Zn2+). Measuring range: 4 -1,000 μg/Dl. Species: all. Sample: serum, plasma, urine, saliva, tissue extract, cell lysate, biological fluid, food, beverage etc. Apparatus: UV/Vis 96-well reader or spectrophotometer. Wavelength: 560 nm (550-580nm). Procedure: 10 min

RayBiotech Mpox (formerly known as monkeypox) Virus (MPXV) PCR Nucleic Acid Detection Kit is a real-time PCR assay for the detection of MPXV DNA in a liquid sample. It uses a fluorescent probe with specific primer sets to detect the J2R gene specific for the genome of MPXV.

The Spike S1 RBD (B.1.1.529, Omicron Variant) (SARS-CoV-2): ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors or neutralizing antibodies of the interaction between the Omicron variant SARS-CoV-2 Spike RBD and human ACE2. The assay requires only a few steps. First, SARS-CoV-2 Spike RBD (B.1.1.529, Omicron Variant) is coated on a 96-well plate overnight. After washing and blocking, the protein is pre-incubated with an inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-ACE2, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can be quenched and measured using a UV/Vis microplate reader.

Protein-protein crosslinking can be achieved by the hydrazone method and thiol-maleimide method. <br /> Thiol-maleimide method is commonly performed with a bifunctional linker, such as the commonly used SMCC. However, SMCC-modified protein is extremely unstable and often self-reactive since proteins often contain both amine and thiol groups that cause a significant amount of homo-crosslinking. The maleimide conjugates can re-arrange, hydrolyze, or exchange in the presence of thiols/cysteine. <br /> <br /> In the hydrazone coupling method, the conjugation reaction is based on a reaction between hydrazine and aldehyde. This method is low yielding although a catalyst is used. The size of the molecule and inaccessibility of the functional group contribute to low yield. In addition, linkage stability is an issue. <br /> <br /> The novel technology of BroadPharm's MagicLink&trade; has been engineered to solve all the problems above and allow rapid and quantifiable conjugation of all classes

High-throughput isolation of genomic DNA from a variety of plant tissue samples in 384-well format.

High-throughput isolation of genomic DNA from a variety of plant tissue samples in 384-well format.