Assays & Kits

HRP can be conjugated to antibody/protein by thiol-maleimide and hydrazone coupling approaches. The thiol-maleimide approach is commonly performed with a bi- functional linker (such as SMCC). However, SMCC-modified protein is extremely unstable and often self-reactive since it often contains both amine and thiol groups that cause a significant amount of homo-crosslinking. The conjugation linkage is exchangeable when other thiol groups are present. In the hydrazone coupling approach, the conjugation reaction is based on a reaction between hydrazine and aldehyde. This method is low yielding although a catalyst is used. The size of the molecule and inaccessibility of the functional group contribute to low yield. In addition, linkage stability is an issue. <br /> <br /> BroadPharm's MagicLink&trade; technology solves all the problems by combining PEG and proprietary crosslinking chemistry. MagicLink&trade;chemistry and kit do not need TCEP, DTT, or catalysts at all!<br /> First, antibody r

HRP can be conjugated to antibody/protein by thiol-maleimide and hydrazone coupling approaches. The thiol-maleimide approach is commonly performed with a bi- functional linker (such as SMCC). However, SMCC-modified protein is extremely unstable and often self-reactive since it often contains both amine and thiol groups that cause a significant amount of homo-crosslinking. The conjugation linkage is exchangeable when other thiol groups are present. In the hydrazone coupling approach, the conjugation reaction is based on a reaction between hydrazine and aldehyde. This method is low yielding although a catalyst is used. The size of the molecule and inaccessibility of the functional group contribute to low yield. In addition, linkage stability is an issue. <br /> <br /> BroadPharm's MagicLink&trade; technology solves all the problems by combining PEG and proprietary crosslinking chemistry. MagicLink&trade; chemistry and kit do not need TCEP, DTT, or catalysts at all!<br /> First, antibody

Oligonucleotide can be conjugated to antibody/protein by thiol-maleimide and hydrazone coupling approaches. The thiol-maleimide approach is commonly performed with a bifunctional linker (such as SMCC). However, SMCC-modified protein is extremely unstable and often self-reactive since it often contains both amine and thiol groups that cause a significant amount of homo-crosslinking. The conjugation linkage is exchangeable when other thiol groups are present. In the hydrazone coupling approach, the conjugation reaction is based on a reaction between hydrazine and aldehyde. This method is low yielding although a catalyst is used. The size of the molecule and inaccessibility of the functional group contribute to low yield. In addition, linkage stability is an issue. <br /> <br /> BroadPharm's MagicLink&trade; technology solves all the problems by combining PEG and proprietary crosslinking chemistry. MagicLink&trade; chemistry and kit do not need TCEP, DTT, or catalysts at all!<br /> First,

Oligonucleotide can be conjugated to antibody/protein by thiol-maleimide and hydrazone coupling approaches. The thiol-maleimide approach is commonly performed with a bifunctional linker (such as SMCC). However, SMCC-modified protein is extremely unstable and often self-reactive since it often contains both amine and thiol groups that cause a significant amount of homo-crosslinking. The conjugation linkage is exchangeable when other thiol groups are present. In the hydrazone coupling approach, the conjugation reaction is based on a reaction between hydrazine and aldehyde. This method is low yielding although a catalyst is used. The size of the molecule and inaccessibility of the functional group contribute to low yield. In addition, linkage stability is an issue. <br /> <br /> BroadPharm's MagicLink&trade; technology solves all the problems by combining PEG and proprietary crosslinking chemistry. MagicLink&trade; chemistry and kit do not need TCEP, DTT, or catalysts at all!<br /> First,

Streptavidin can be conjugated to antibody/protein by thiol-maleimide and hydrazone coupling approaches. The thiol-maleimide approach is commonly performed with a bifunctional linker (such as SMCC). However, SMCC-modified protein is extremely unstable and often self-reactive since it often contains both amine and thiol groups that cause a significant amount of homo-crosslinking. The conjugation linkage is exchangeable when other thiol groups are present.<br /> <br /> In the hydrazone coupling approach, the conjugation reaction is based on a reaction between hydrazine and aldehyde. This method is low yielding although a catalyst is used. The size of the molecule and inaccessibility of the functional group contribute to low yield. In addition, linkage stability is an issue. <br /> <br /> BroadPharm's MagicLink&trade; technology solves all the problems by combining PEG and proprietary crosslinking chemistry. MagicLink&trade;chemistry and kit and do not need TCEP, DTT, or catalysts.<br />

Protein-protein crosslinking can be achieved by the hydrazone method and thiol-maleimide method. <br /> Thiol-maleimide method is commonly performed with a bifunctional linker, such as the commonly used SMCC. However, SMCC-modified protein is extremely unstable and often self-reactive since proteins often contain both amine and thiol groups that cause a significant amount of homo-crosslinking. The maleimide conjugates can re-arrange, hydrolyze, or exchange in the presence of thiols/cysteine. <br /> <br /> In the hydrazone coupling method, the conjugation reaction is based on a reaction between hydrazine and aldehyde. This method is low yielding although a catalyst is used. The size of the molecule and inaccessibility of the functional group contribute to low yield. In addition, linkage stability is an issue. <br /> <br /> The novel technology of BroadPharm's MagicLink&trade; has been engineered to solves all the problems above and allow rapid and quantifiable conjugation of all classes

The fluorescein labeling kits are high-performance derivatives of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.<br /> Fluorescein dyes are used in wide-ranging applications including fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western Blotting and ELISA. Fluorescein NHS are reactive toward primary amine groups on proteins, peptides and other biomolecules, while fluorescein--maleimide react with free sulfhydryl on cysteine residues. <br /> <br /> Most activated fluorescein derivatives are mixtures of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. There is a big difference in flow cytometry although it is a small difference in the properties of these isomers in terms of excitation and emission spectra. The small difference will make your assay complicated because the ratio of two isomers varies lot by lot. <br />BroadPharm flu

The fluorescein labeling kits are high-performance derivatives of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.<br /> Fluorescein dyes are used in wide-ranging applications including fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western Blotting and ELISA. Fluorescein NHS are reactive toward primary amine groups on proteins, peptides and other biomolecules, while fluorescein--maleimide react with free sulfhydryl on cysteine residues. <br /> <br /> Most activated fluorescein derivatives are mixtures of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. There is a big difference in flow cytometry although it is a small difference in the properties of these isomers in terms of excitation and emission spectra. The small difference will make your assay complicated because the ratio of two isomers varies lot by lot. <br />BroadPharm flu

The fluorescein labeling kits are high-performance derivatives of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.<br /> Fluorescein dyes are used in wide-ranging applications including fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western Blotting and ELISA. Fluorescein NHS are reactive toward primary amine groups on proteins, peptides and other biomolecules, while fluorescein--maleimide react with free sulfhydryl on cysteine residues. <br /> <br /> Most activated fluorescein derivatives are mixtures of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. There is a big difference in flow cytometry although it is a small difference in the properties of these isomers in terms of excitation and emission spectra. The small difference will make your assay complicated because the ratio of two isomers varies lot by lot. <br />BroadPharm flu