Assays & Kits

800CW (IRDye 800CW acid equivalent) is a near-infrared fluorescent dyes. The unique structure has 4 sulfonates which make it as a high water soluble and less aggregation in aqueous solution. 800CW is used for protein and antibody labeling, or nucleic acid applications with high labeling density.<br /> 800CW dye-conjugated agents and probes are currently propelling more than a dozen Phase I or Phase II clinical trials, more than any other near-infrared fluorescent dye based on its enhanced sensitivity due to low background autofluorescence in the near-infrared region and, therefore, higher signal-to-noise ratios.

800CW (IRDye 800CW acid equivalent) is a near-infrared fluorescent dyes. The unique structure has 4 sulfonates which make it as a high water soluble and less aggregation in aqueous solution. 800CW is used for protein and antibody labeling, or nucleic acid applications with high labeling density.<br /> 800CW dye-conjugated agents and probes are currently propelling more than a dozen Phase I or Phase II clinical trials, more than any other near-infrared fluorescent dye based on its enhanced sensitivity due to low background autofluorescence in the near-infrared region and, therefore, higher signal-to-noise ratios.

MagicLink&trade; Sulfo-NHS-Biotin is a water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.<br /> Sulfo-NHS-Biotin is the shortest arm length (13.5 angstroms) of three MagicLink&trade; biotinylation reagent which is water-soluble, non-cleavable, and enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents. Differing only in their spacer arm lengths, the Sulfo-NHS-ester reagents offer the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Sulfo-NHS-Biotin is offered in separate package sizes and as complete protein labeling kits.<br /> <Br>Features: <ul style="list-style-type: disc"><li><strong>Amine-reactive:</strong

BroadPharm MagicLink&trade; Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines. <br>Features: <ul style="list-style-type: disc"><li><strong>Amine-reactive:</strong> reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides</li><li><strong>Soluble charged:</strong> sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds</li><li><strong>Medium length spacer arm:</strong> (total length added to target) is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass</li><li><strong>Irreversible forms:</strong> permanent amide bonds; spacer arm cannot be cleaved</li><li><strong>Cell surface:</strong> labeling biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes</li><li><

The BroadPharm Biotin Quantitation kit enables a quick estimation of the mole-to-mole ratio of biotin to protein. To quantify biotin label incorporation, a solution containing the biotinylated protein is added to a mixture of HABA and avidin. Because of its higher affinity for avidin, biotin displaces HABA from its interaction with avidin and the absorption at 500 nm decreases proportionately. By this method, an unknown amount of biotin present in a solution can be evaluated in a single cuvette by measuring the absorbance of the HABA-avidin solution before and after addition of the biotin-containing sample. The change in absorbance relates to the amount of biotin in the sample.<br /> <br /> For your biotinylating needs. refer to BroadPharm kits Sulfo-NHS-LC-Biotin kit and reagent (BP-50058), Sulfo-NHS-Biotin (BP-50056), and NHS-PEG4-Biotin (BP-50054)

HRP can be conjugated to antibody/protein by thiol-maleimide and hydrazone coupling approaches. The thiol-maleimide approach is commonly performed with a bi- functional linker (such as SMCC). However, SMCC-modified protein is extremely unstable and often self-reactive since it often contains both amine and thiol groups that cause significant amounts of homo-crosslinking. The thiol-maleimide linkage is exchangeable when other thiol groups are present. In the hydrazone coupling approach, the conjugation reaction is based on a reaction between hydrazine and aldehyde. This method is low yielding although a catalyst is used. The size of the molecule and inaccessibility of the functional group contribute to low yield. In addition, linkage stability is an issue. <br /> <br /> BroadPharm's MagicLink&trade; technology solves all the problems by combining PEG and proprietary crosslinking chemistry. MagicLink&trade; chemistry and kit do not need TCEP, DTT, or catalysts at all!<br /> First, antibo

HRP can be conjugated to antibody/protein by thiol-maleimide and hydrazone coupling approaches. The thiol-maleimide approach is commonly performed with a bi- functional linker (such as SMCC). However, SMCC-modified protein is extremely unstable and often self-reactive since it often contains both amine and thiol groups that cause significant amounts of homo-crosslinking. The thiol-maleimide linkage is exchangeable when other thiol groups are present. In the hydrazone coupling approach, the conjugation reaction is based on a reaction between hydrazine and aldehyde. This method is low yielding although a catalyst is used. The size of the molecule and inaccessibility of the functional group contribute to low yield. In addition, linkage stability is an issue. <br /> <br /> BroadPharm's MagicLink&trade; technology solves all the problems by combining PEG and proprietary crosslinking chemistry. MagicLink&trade; chemistry and kit do not need TCEP, DTT, or catalysts at all!<br /> First, antibo

MagicLink&trade; Site Specific Biotinylation Kits provide optimized reagents for biotinylating antibodies. Biotin is a small, naturally occurring vitamin that binds with high affinity to avidin and avidin-like proteins. Biotinylated antibodies typically retain biological activity because the biotin group is relatively small. Current bintinylation methods, such as amine-NHS ester and converting amine to thiol and then use maleimide chemistry, will produce random biotin on the antibody. In some cases, labeling in some Fab areas could block antigens binding. With site specific biotinylation chemistry, biotin linkers are inserted to thiol-bridge locations and do not affect antibody-antigen binding (Fig.1). It will help increase the sensitivity of bio assay such as ELISA, Western Blot, etc. </p> This conjugation reaction is simple, site-specific, and efficient. These kits include biotin linker, reaction buffer, protein concentrator, DMSO, and TCEP. The user can follow easy protocol and get

MagicLink&trade; Site Specific Biotinylation Kits provide optimized reagents for biotinylating antibodies. Biotin is a small, naturally occurring vitamin that binds with high affinity to avidin and avidin-like proteins. Biotinylated antibodies typically retain biological activity because the biotin group is relatively small. Current bintinylation methods, such as amine-NHS ester and converting amine to thiol and then use maleimide chemistry, will produce random biotin on the antibody. In some cases, labeling in some Fab areas could block antigens binding. With site specific biotinylation chemistry, biotin linkers are inserted to thiol-bridge locations and do not affect antibody-antigen binding (Fig.1). It will help increase the sensitivity of bio assay such as ELISA, Western Blot, etc. </p> This conjugation reaction is simple, site-specific, and efficient. These kits include biotin linker, reaction buffer, protein concentrator, DMSO, and TCEP. The user can follow easy protocol and get