Assays & Kits

BroadPharm's SN-38 Antibody Conjugation kit attaches the drug, SN-38 to antibody. The kit is designed to utilize the linker's maleimide group to react and attach to thiol groups on the antibody.</p> The linker- SN-38 is made by SN38 conjugated to the lysosomally cleavable dipeptide, valine-citrulline (vc) linker. SN-38 is the active metabolite of the topoisomerase-I (topo-I) inhibitor Irinotecan (CPT-11 ), and it stabilizes the complex between topo-I and DNA which collide with moving DNA replication forks, eventually leading to double stranded DNA damage.</p> <ol type='A'> Kit Features: <li>Protocol: easy to follow, to label IgG with SN38 with minimum exposure to the chemotherapeutic drug</li> <li>Linkage: releasable linkage</li> <li>Fast process: <1 h hands-on time, 6 h for whole process. </li> <li>Convient: All reagents and supplies included for preparation purification, and storage</li> <li>DAR: average 3-8</li> <li>Efficient: More than 99% conjugated products by SEC and is f

BroadPharm's SN-38 Antibody Conjugation kit attaches the drug, SN-38 to antibody. The kit is designed to utilize the linker's maleimide group to react and attach to thiol groups on the antibody.</p> The linker- SN-38 is made by SN38 conjugated to the lysosomally cleavable dipeptide, valine-citrulline (vc) linker. SN-38 is the active metabolite of the topoisomerase-I (topo-I) inhibitor Irinotecan (CPT-11 ), and it stabilizes the complex between topo-I and DNA which collide with moving DNA replication forks, eventually leading to double stranded DNA damage.</p> <ol type='A'> Kit Features: <li>Protocol: easy to follow, to label IgG with SN38 with minimum exposure to the chemotherapeutic drug</li> <li>Linkage: releasable linkage</li> <li>Fast process: <1 h hands-on time, 6 h for whole process. </li> <li>Convient: All reagents and supplies included for preparation purification, and storage</li> <li>DAR: average 3-8</li> <li>Efficient: More than 99% conjugated products by SEC and is f

The PCSK9(D374T) [Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. The key to this kit is the high sensitivity of detection of biotin-labeled PCSK9(D374T) by streptavidin-HRP. Only a few steps on a microtiter plate are required for the assay. First, LDLR ectodomain is coated on a 96-well plate. Next, PCSK9(D374T) is incubated with LDLR on the plate.  Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

The PCSK9(D374T) [Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. The key to this kit is the high sensitivity of detection of biotin-labeled PCSK9(D374T) by streptavidin-HRP. Only a few steps on a microtiter plate are required for the assay. First, LDLR ectodomain is coated on a 96-well plate. Next, PCSK9(D374T) is incubated with LDLR on the plate.  Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit does not include E. coli cells; however, another version of the kit includes E.coli chemically competent cells for transformation (BPS Bioscience #78532).

The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit does not include E. coli cells; however, another version of the kit includes E.coli chemically competent cells for transformation (BPS Bioscience #78532).

The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).

The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).

The PARP2 Homogeneous Assay Kit is designed to measure PARP2 activity for screening and profiling applications. PARP2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The PARP2 Homogeneous Assay Kit takes advantage of a highly specific antibody that recognizes the PARylated form of the substrate. With this kit, only three simple steps are required for PARP2 reactions. First, a sample containing PARP2 enzyme is incubated with biotinylated substrate and NAD for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.