Assays & Kits

The Chemi-Verse™ Aurora Kinase C Assay Kit is a luminescence assay designed to measure Aurora Kinase C activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant Aurora Kinase C, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ MEK1 Kinase Assay Kit is a luminescent assay designed to measure MEK1 (dual specificity mitogen-activated protein kinase kinase 1) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant MEK1 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

This kit is used for the fast quantification of Trastuzumab/Herceptin® (Monkey) in serum,plasma and cell supernatant samples. (Homogenous Assays, no wash step, quantitative assay)

CISH Implementation Kit 2 is intended to be used in combination with digoxigenin/dinitrophenyl-labeled CISH probes for the qualitative detection of genetic abbreviations, e.g., translocations, deletions, amplifications, and chromosome aneuploidies, in formalin-fixed, paraffin-embedded specimens by chromogenic <i>in situ</i> hybridization (CISH).

The Ig-kappa/Ig-lambda (Human) CISH Kit is intended to be used for the simultaneous qualitative detection of human Ig-kappa and Ig-lambda light chain mRNA in formalin-fixed, paraffin-embedded specimens, such as lymphomas, by CISH.

The AP-NBT/BCIP CISH Implementation Kit is intended to be used for the qualitative detection of digoxigenin-labeled CISH Probes in CISH.

The AP-Permanent Red CISH Implementation Kit is intended to be used for the qualitative detection of digoxigenin-labeled CISH Probes in CISH.

The DNA Polymerase θ Assay Kit is a fluorogenic assay designed to measure DNA Polymerase θ activity for screening and profiling applications, utilizing the fluorescent nucleic acid dye GroovyGreen™ for the quantitation of double-stranded DNA (dsDNA) products. The kit comes in a convenient 96-well format and contains enough recombinant DNA Polymerase θ enzyme (amino acids 1792-2590), DR Substrate 3, dNTP mix and buffer for 100 enzymatic reactions.  DNA Polymerase θ uses available nucleotides to synthesize a DNA strand complementary to the template strand, leading to the formation of double-stranded DNA molecules (dsDNA). The amount of dsDNA formed directly correlates to the polymerase activity and can be quantified by the addition of a fluorescent dsDNA-binding dye which distinguishes dsDNA from ssDNA and free nucleotides. Since the dye emits fluorescence only when bound to the target dsDNA, the fluorometric readouts are low in the presence of a polymerase inhibitor.  

Human AhR activation and cell viability in 96 well format. This product is available for laboratory services; please contact your local office to discuss and build your project.