Assays & Kits

The Chemi-Verse™ BTK Kinase Assay Kit is designed to measure BTK (Bruton's tyrosine kinase) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified BTK, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The MLL1 (KMT2A): WDR5 Binding Chemiluminescent Assay Kit is an ELISA-based assay designed to measure the binding between MLL1 (mixed lineage leukemia protein-1, also known as KMT2A) and WDR5 (WD40 repeat-containing protein 5) for screening and profiling applications. The MLL1 (KMT2A): WDR5 Binding Chemiluminescent Assay Kit comes with enough purified MLL1 (amino acids 3745-end) and WDR5 proteins, primary and secondary antibody, assay buffer, and detection reagent for 100 enzyme reactions. A 96-well plate is coated with MLL1 protein. After coating and blocking, WDR5 is added in an optimized assay buffer. Unbound WDR5 is washed away, and the plate is incubated with a primary antibody followed by a secondary HRP-conjugated antibody. Finally, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of WDR5 binding to MLL1 (KMT2A).

The MLL4 (KMT2B): WDR5 Binding Chemiluminescent Assay Kit is an ELISA-based assay designed to measure the binding between MLL4 (mixed lineage leukemia protein-4, also known as KMT2B) and WDR5 (WD40 repeat-containing protein 5) for screening and profiling applications. The MLL4 (KMT2B): WDR5 Binding Chemiluminescent Assay comes with enough purified MLL4 (amino acids 2490-2715) and WDR5 proteins, primary and secondary antibody, assay buffer, and detection reagent for 100 enzyme reactions. A 96-well plate is coated with MLL4 protein. After coating and blocking, WDR5 is added in an optimized assay buffer. Next, unbound WDR5 is washed away, and the plate is incubated with a primary antibody followed by a secondary HRP-conjugated antibody. Finally, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of WDR5 binding to MLL4 (KMT2B).

The Chemi-Verse™ EGFR Kinase Assay Kit is designed to measure EGFR (epidermal growth factor receptor) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough recombinant purified EGFR (amino acids 668-1210), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The TSLPR:TSLP [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling molecules that block the binding between TSLPR (thymic stromal lymphopoietin receptor) and TSLP. This kit comes in a convenient 96-well format, with enough recombinant human biotin-labeled TSLP (amino acids 29-159), human TSLP-R (amino acids 25-231), streptavidin-HRP, and assay buffer for 100 reactions. A 96-well plate  is coated with TSLPR protein. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-TSLP, the plate is washed and Streptavidin-HRP is added. The ELISA ECL substrate is added  and the resulting signal can be measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of TSLPR to TSLP.

The BLM Helicase Activity Assay Kit is a fluorogenic assay designed for screening and profiling of BLM (Bloom syndrome protein) antagonists/inhibitors by monitoring their inhibitory effects on BLM-catalyzed DNA unwinding. The BLM Helicase Activity Assay Kit comes in a convenient 96-well format, with enough purified recombinant BLM (amino acids 630-1300), ATP, DNA substrate, assay buffer, and additives for 100 reactions. The DNA substrate is conjugated on one strand with the TAMRA (tetramethylrhodamine) fluorophore, and on the other strand with BHQ (Black Hole Quencher) which effectively quenches TAMRA fluorescence due to their proximity within the DNA double strand. BLM unwinding of the DNA probe separates the two strands, releasing TAMRA fluorescence. BLM activity, therefore, results in a proportional increase in fluorescence.

"The CUTANA™ Quick Cleanup DNA Purification Kit can be used to purify CUT&RUN or CUT&Tag DNA and remove unwanted adapter-/primer-dimers from these reactions. Unlike many kits that purify DNA using spin columns, this kit uses SPRI paramagnetic beads for streamlined, higher throughput workflows easily integrated into CUTANA™ genomic mapping assays. Depending on the application, various SPRI bead:DNA ratios are added to capture target DNA. In CUT&RUN, pAG-MNase cleaves DNA proximal to antibody-labeled chromatin, releasing it into the supernatant. It is imperative to recover the highest DNA yield possible from the supernatant; therefore, a higher bead:DNA ratio is added to span a wide range of DNA fragment lengths. This kit includes sufficient volume to perform 48 total CUT&RUN reactions at an empirically optimized higher bead ratio. In CUT&Tag, pAG-Tn5 cleaves antibody-bound chromatin and simultaneously ligates adapter DNA, resulting in longer DNA fragments. Further, in the EpiCypher C

Cytoplasmic isocitrate dehydrogenase (ICDHc, EC 1.1.1.42) is widely distributed in animals, plants, microorganisms, and cultured cells. It catalyzes the decarboxylation and dehydrogenation of isocitrate to produce α-ketoglutarate, while simultaneously reducing NADP+ to NADPH. ICDHc serves as another significant source of NADPH in the cytoplasm, apart from the pentose phosphate pathway, and its activity often undergoes notable changes under stress conditions. CheKine™ Micro Isocitrate Dehydrogenase Cytoplasmic (ICDHc) Activity Assay Kit is capable of detecting cytosolic isocitrate dehydrogenase (ICDHc) activity in animal and plant tissues, cells, or bacteria, as well as in serum (plasma). The principle behind this involves utilizing ICDHc's catalytic action in reducing NADP+ to NADPH, with the subsequent increase in NADPH concentration being measured at a wavelength of 340 nm.

β-glucuronidase (β-GD), a matrix degradation enzyme involved in tumor invasion and metastasis, is widely found in animal tissues. It has physiological functions such as hydrolyzing sterol glucuronic acid and acidic mucopolysaccharide. The content of this enzyme is high in hepatocytes. In addition, it is rich in gastric cancer, so the determination of β-GD activity in gastric juice is of great significance for the study of gastric cancer. CheKine™ Micro β-glucuronidase (β-GD) Activity Assay Kit can detect animal tissues. In this kit, β-GD catalyzed phenol β-D-glucuronic acid to produce free phenolphthalein, and the enzyme activity was determined by determining the content of phenol.